Click image to see more details
Product Info Summary
| SKU: | PA1549 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-CD89/FCAR Antibody Picoband®
SKU/Catalog Number
PA1549
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CD89/FCAR Antibody catalog # PA1549. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD89/FCAR Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1549)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle of human CD89.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1549 is reactive to FCAR in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
70 kDa
Calculated molecular weight
32265 MW
Background of FCAR/CD89
FCAR, Receptor for Fc fragment of IGA, is also known as CD89. Human Fc-alpha receptor (FCAR) is present on a number of cell types, including neutrophils, monocytes, macrophages, and eosinophils. FCAR interacts with aggregated IgAs, such as IgA coated on the surface of an invading microorganism, and mediates several immunologic defense processes such as phagocytosis, antibody-dependent cell-mediated cytotoxicity, and stimulation of the release of inflammatory mediators. FCAR is a glycoprotein of 50 to 100 kD, with diversity on different cell types. FCAR is mapped to 19q13.4. Human COS cells transfected with FCAR cDNA bind to IgA, but not IgG.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1549 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: A549 Cell, U87 Cell, RAJI Cell, JURKAT Cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of CD89/FCAR using anti-CD89/FCAR antibody (PA1549).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human K562 whole cell lysates,
Lane 2: human Jurkat whole cell lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: human CACO-2 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD89/FCAR antigen affinity purified polyclonal antibody (Catalog # PA1549) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-DyLight 647 Conjugated secondary antibody (Left) at a dilution of 1:2000 or a goat anti-rabbit IgG-HRP Conjugated secondary antibody (Right) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD89/FCAR approximately 70 kDa. The expected band size for CD89/FCAR is at 32 kDa.
Protein Target Info & Infographic
Gene/Protein Information For FCAR (Source: Uniprot.org, NCBI)
Gene Name
FCAR
Full Name
Immunoglobulin alpha Fc receptor
Weight
32265 MW
Alternative Names
Immunoglobulin alpha Fc receptor;IgA Fc receptor;CD89;FCAR;CD89; FCAR CD89, CTB-61M7.2, FcalphaRI Fc fragment of IgA receptor immunoglobulin alpha Fc receptor|FCAR variant 14|Fc alpha receptor|Fc fragment of IgA, receptor for
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on FCAR, check out the FCAR Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for FCAR: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CD89/FCAR Antibody Picoband® (PA1549)
Hello CJ!
No publications found for PA1549
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-CD89/FCAR Antibody Picoband®?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-CD89/FCAR Antibody Picoband®
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
1 Customer Q&As for Anti-CD89/FCAR Antibody Picoband®
Question
We are currently using anti-CD89/FCAR antibody PA1549 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on dog tissues as well?
A. Singh
Verified customer
Asked: 2014-01-30
Answer
The anti-CD89/FCAR antibody (PA1549) has not been tested for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2014-01-30
