Anti-CD45RB (B-Cell Marker) PTPRC Monoclonal Antibody

Product Name

Anti-CD45RB (B-Cell Marker) PTPRC Monoclonal Antibody

View all PTPRC Antibodies

SKU/Catalog Number

M00555

Size

100ug/vial

Form

Liquid

Description

Boster Bio Anti-CD45RB (B-Cell Marker) PTPRC Monoclonal Antibody (Catalog # M00555). Tested in Flow Cytometry, IF, WB, IHC applications. This antibody reacts with Human.

Storage & Handling

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Cite This Product

Anti-CD45RB (B-Cell Marker) PTPRC Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00555)

Host

Mouse

Contents

Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Clonality

Monoclonal

Clone Number

Clone: BRA-11; same as BRA-11G

Isotype

IgG1, kappa

Immunogen

Non-T, non-B CALLA positive ALL cell line REH (Leucocyte Workshop IV and V)

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Does not cross-react with primate, avian or amphibian GR.

Reactive Species

M00555 is reactive to PTPRC in Human

Applications

M00555 is guaranteed for Flow Cytometry, IF, IHC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

147254 MW

Background of PTPRC

CD45R, also designated CD45 and PTPRC, has been identified as a transmembrane glycoprotein, broadly expressed among hematopoietic cells. Multiple isoforms of CD45R are distributed throughout the immune system according to cell type. These isoforms arise because of alternative splicing of exons 4, 5, and 6. The corresponding protein domains are characterized by the binding of monoclonal antibodies specific for CD45RA (exon 4), CD45RB (exon 5), CD45RC (exon 6) and CD45RO (exons 4 to 6 spliced out). The variation in these isoforms is localized to the extracellular domain of CD45R, while the intracellular domain is conserved. CD45RB is expressed on mature B-lymphocytes and the majority of lymphomas and leukemias of B-cell origin.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconstitution

Reconstitute with distilled water.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Validation Images & Assay Conditions

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Formalin-fixed, paraffin-embedded human Tonsil stained with Anti-CD45RB Mouse Monoclonal Antibody (BRA-11).

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Western Blot analysis of human Raji cell lysate using Anti-CD45RB Mouse Monoclonal Antibody (BRA-11).

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Flow Cytometric analysis of PFA-fixed Raji cells using Anti-CD45RB Mouse Monoclonal Antibody (BRA-11) followed by Goat anti- Mouse- IgG-CF488 (Blue); Isotype Control (Red).

Gene/Protein Information For PTPRC (Source: Uniprot.org, NCBI)

Alternative Names

B220; CD45 antigen; CD45; CD45R; EC 3.1.3.48; LCA; L-CA; LY5; protein tyrosine phosphatase, receptor type, C; PTPRC; receptor-type tyrosine-protein phosphatase C; T200 Glycoprotein; T200 leukocyte common antigen; T200receptor type, c polypeptide PTPRC B220, CD45, CD45R, GP180, L-CA, LCA, LY5, T200 protein tyrosine phosphatase receptor type C receptor-type tyrosine-protein phosphatase C|CD45 antigen|T200 glycoprotein|T200 leukocyte common antigen|protein tyrosine phosphatase, receptor type, c polypeptide

For more info on PTPRC, check out the PTPRC Infographic

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In this infographic, you will see the following information for PTPRC: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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