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Product Info Summary
| SKU: | M00555-2 |
|---|---|
| Size: | 100uL |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-CD45 PTPRC Rabbit Monoclonal Antibody, Clone#RM291
SKU/Catalog Number
M00555-2
Size
100uL
Form
Liquid
Description
Boster Bio Anti-CD45 PTPRC Rabbit Monoclonal Antibody, Clone#RM291 (Catalog # M00555-2). Tested in IHC, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD45 PTPRC Rabbit Monoclonal Antibody, Clone#RM291 (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00555-2)
Host
Rabbit
Contents
50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Clonality
Monoclonal
Clone Number
RM291
Isotype
Rabbit IgG
Immunogen
A peptide corresponding to the C-terminus of human CD45 (Receptor-type tyrosine-protein phosphatase C).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
This antibody reacts to human CD45 (Receptor-type tyrosine-protein phosphatase C).
Reactive Species
M00555-2 is reactive to PTPRC in Human
Reconstitution
Calculated molecular weight
147254 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00555-2 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (IHC): 1:100-1:200 dilution
WB: 1:1000-1:2000 dilution.
Validation Images & Assay Conditions
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Figure 1. Western Blotting result
Western Blot of Jurkat cell lysates using anti-CD45 rabbit monoclonal antibody (clone RM291) at a 1:2,000 dilution.
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Figure 2. IHC result
Immunohistochemical staining of formalin fixed and paraffin embedded human tonsil tissue sections using anti-CD45 rabbit monoclonal antibody (clone RM291) at a 1:200 dilution.
Protein Target Info & Infographic
Gene/Protein Information For PTPRC (Source: Uniprot.org, NCBI)
Gene Name
PTPRC
Full Name
Receptor-type tyrosine-protein phosphatase C
Weight
147254 MW
Superfamily
protein-tyrosine phosphatase family
Alternative Names
Receptor-type tyrosine-protein phosphatase C;3.1.3.48;Leukocyte common antigen;L-CA;T200;CD45;PTPRC;CD45; PTPRC B220, CD45, CD45R, GP180, L-CA, LCA, LY5, T200 protein tyrosine phosphatase receptor type C receptor-type tyrosine-protein phosphatase C|CD45 |T200 glycoprotein|T200 leukocyte common |protein tyrosine phosphatase, receptor type, c polypeptide
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PTPRC, check out the PTPRC Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PTPRC: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CD45 PTPRC Rabbit Monoclonal Antibody, Clone#RM291 (M00555-2)
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No publications found for M00555-2
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-CD45 PTPRC Rabbit Monoclonal Antibody, Clone#RM291
Question
I have attached the WB image, lot number and protocol we used for liver using anti-CD45 Rabbit Monoclonal antibody, Clone#RM291 M00555-2. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-03-30
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-03-30
Question
I see that the anti-CD45 Rabbit Monoclonal antibody, Clone#RM291 M00555-2 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-02-04
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-02-04
Question
I was wanting to use your anti-CD45 Rabbit Monoclonal antibody, Clone#RM291 for IHC for human liver on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human liver identification?
Verified Customer
Verified customer
Asked: 2020-01-07
Answer
You can see on the product datasheet, M00555-2 anti-CD45 Rabbit Monoclonal antibody, Clone#RM291 has been tested for IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human liver in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-01-07
Question
Our team were well pleased with the WB result of your anti-CD45 Rabbit Monoclonal antibody, Clone
Verified Customer
Verified customer
Asked: 2019-12-31
Answer
From what I have seen in literature, leukemic t-cell does express PTPRC. Generally PTPRC expresses in cell membrane. Regarding which tissues have PTPRC expression, here are a few articles citing expression in various tissues:
Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19349973, 19690332
Liver, Pubmed ID: 19159218
Lymphocyte, Pubmed ID: 2824653
Placenta, Pubmed ID: 2971730, 2845400
Synovium, Pubmed ID: 14702039
T-cell, Pubmed ID: 19367720
Boster Scientific Support
Answered: 2019-12-31
Question
Is there a BSA free version of anti-CD45 Rabbit Monoclonal antibody, Clone#RM291 M00555-2 available?
Verified Customer
Verified customer
Asked: 2019-10-24
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-CD45 Rabbit Monoclonal antibody, Clone#RM291 M00555-2 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-10-24
Question
Does anti-CD45 Rabbit Monoclonal antibody, Clone#RM291 M00555-2 work for IHC with liver?
Verified Customer
Verified customer
Asked: 2019-08-23
Answer
According to the expression profile of liver, PTPRC is highly expressed in liver. So, it is likely that anti-CD45 Rabbit Monoclonal antibody, Clone#RM291 M00555-2 will work for IHC with liver.
Boster Scientific Support
Answered: 2019-08-23
Question
We have observed staining in human blood. Are there any suggestions? Is anti-CD45 Rabbit Monoclonal antibody, Clone#RM291 supposed to stain blood positively?
T. Huang
Verified customer
Asked: 2019-08-22
Answer
From literature blood does express PTPRC. From Uniprot.org, PTPRC is expressed in blood, lymphocyte, synovium, placenta, t-cell, liver, leukemic t-cell, erythroleukemia, among other tissues. Regarding which tissues have PTPRC expression, here are a few articles citing expression in various tissues:
Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19349973, 19690332
Liver, Pubmed ID: 19159218
Lymphocyte, Pubmed ID: 2824653
Placenta, Pubmed ID: 2971730, 2845400
Synovium, Pubmed ID: 14702039
T-cell, Pubmed ID: 19367720
Boster Scientific Support
Answered: 2019-08-22
Question
My lab would like using your anti-CD45 Rabbit Monoclonal antibody, Clone#RM291 for calcium-mediated signaling using intracellular calcium source studies. Has this antibody been tested with western blotting on human tonsil tissue? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-06-14
Answer
We appreciate your inquiry. This M00555-2 anti-CD45 Rabbit Monoclonal antibody, Clone#RM291 is tested on human tonsil tissue, jurkat cell lysates. It is guaranteed to work for IHC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-06-14
Question
I have a question about product M00555-2, anti-CD45 Rabbit Monoclonal antibody, Clone#RM291. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-05-14
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00555-2 anti-CD45 Rabbit Monoclonal antibody, Clone#RM291, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-05-14
Question
Our lab used your anti-CD45 Rabbit Monoclonal antibody, Clone
Verified Customer
Verified customer
Asked: 2019-05-09
Answer
I looked at the website and datasheets of our anti-CD45 Rabbit Monoclonal antibody, Clone
Boster Scientific Support
Answered: 2019-05-09
Question
We are interested in to test anti-CD45 Rabbit Monoclonal antibody, Clone#RM291 M00555-2 on human liver for research purposes, then I may be interested in using anti-CD45 Rabbit Monoclonal antibody, Clone#RM291 M00555-2 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-12-04
Answer
The products we sell, including anti-CD45 Rabbit Monoclonal antibody, Clone#RM291 M00555-2, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-12-04
Question
Is a blocking peptide available for product anti-CD45 Rabbit Monoclonal antibody, Clone#RM291 (M00555-2)?
Verified Customer
Verified customer
Asked: 2018-09-12
Answer
We do provide the blocking peptide for product anti-CD45 Rabbit Monoclonal antibody, Clone#RM291 (M00555-2). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-09-12
Question
We are currently using anti-CD45 Rabbit Monoclonal antibody, Clone#RM291 M00555-2 for human tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2017-09-25
Answer
The anti-CD45 Rabbit Monoclonal antibody, Clone#RM291 (M00555-2) has not been tested for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-09-25
Question
Is this M00555-2 anti-CD45 Rabbit Monoclonal antibody, Clone#RM291 reactive to the isotypes of PTPRC?
Verified Customer
Verified customer
Asked: 2017-06-07
Answer
The immunogen of M00555-2 anti-CD45 Rabbit Monoclonal antibody, Clone#RM291 is A peptide corresponding to the C-terminus of human CD45 (Receptor-type tyrosine-protein phosphatase C). . Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-06-07
Question
Would M00555-2 anti-CD45 Rabbit Monoclonal antibody, Clone#RM291 work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
P. Jones
Verified customer
Asked: 2016-08-23
Answer
It shows on the product datasheet, M00555-2 anti-CD45 Rabbit Monoclonal antibody, Clone#RM291 as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2016-08-23
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-CD45 Rabbit Monoclonal antibody, Clone#RM291 M00555-2. Let me know if you need anything else.
C. Baker
Verified customer
Asked: 2014-03-17
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2014-03-17
