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Product Info Summary
| SKU: | A01102-1 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, IHC, WB |
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Product info
Product Name
Anti-CD41/Integrin Alpha 2B/ITGA2B Antibody Picoband®
SKU/Catalog Number
A01102-1
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-CD41/Integrin Alpha 2B/ITGA2B Antibody Picoband® catalog # A01102-1. Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-CD41/Integrin Alpha 2B/ITGA2B Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01102-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human CD41/Integrin Alpha 2B/ITGA2B recombinant protein (Position: A675-R714).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01102-1 is reactive to ITGA2B in Human
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
125 kDa
Calculated molecular weight
113.377kDa
Background of ITGA2B
Integrin alpha-IIb is a protein that in humans is encoded by the ITGA2B gene. It is mapped to 17q21.32. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. Alpha chain 2b undergoes post-translational cleavage to yield disulfide-linked light and heavy chains that join with beta 3 to form a fibrinogen receptor expressed in platelets that plays a crucial role in coagulation. Mutations that interfere with this role result in thrombasthenia. In addition to adhesion, integrins are known to participate in cell-surface mediated signalling.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01102-1 is guaranteed for ELISA, Flow Cytometry, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human HEL whole cell, human Hela whole cell
IHC: human spleen tissue
FCM: HEL cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of CD41/Integrin Alpha 2B/ITGA2B using anti-CD41/Integrin Alpha 2B/ITGA2B antibody (A01102-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HEL whole cell lysates,
Lane 2: human Hela whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD41/Integrin Alpha 2B/ITGA2B antigen affinity purified polyclonal antibody (Catalog # A01102-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD41/Integrin Alpha 2B/ITGA2B at approximately 125 kDa. The expected band size for CD41/Integrin Alpha 2B/ITGA2B is at 113 kDa.
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Figure 2. IHC analysis of CD41/Integrin Alpha 2B/ITGA2B using anti-CD41/Integrin Alpha 2B/ITGA2B antibody (A01102-1).
CD41/Integrin Alpha 2B/ITGA2B was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD41/Integrin Alpha 2B/ITGA2B Antibody (A01102-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Figure 3. Flow Cytometry analysis of HEL cells using anti-CD41/Integrin Alpha 2B/ITGA2B antibody (A01102-1).
Overlay histogram showing HEL cells stained with A01102-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-CD41/Integrin Alpha 2B/ITGA2B Antibody (A01102-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For ITGA2B (Source: Uniprot.org, NCBI)
Gene Name
ITGA2B
Full Name
Integrin alpha-IIb
Weight
113.377kDa
Superfamily
integrin alpha chain family
Alternative Names
Putative Polycomb group protein ASXL1; Additional sex combs-like protein 1; ASXL1; KIAA0978 ITGA2B BDPLT16, BDPLT2, CD41, CD41B, GP2B, GPIIb, GT, GTA, HPA3, PPP1R93 integrin subunit alpha 2b integrin alpha-IIb|GPalpha IIb|alphaIIb protein|integrin, alpha 2b (platelet glycoprotein IIb of IIb/IIIa complex, CD41)|platelet fibrinogen receptor, alpha subunit|platelet glycoprotein IIb of IIb/IIIa complex|platelet membrane glycoprotein IIb|platelet-specific BAK|protein phosphatase 1, regulatory subunit 93
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ITGA2B, check out the ITGA2B Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ITGA2B: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CD41/Integrin Alpha 2B/ITGA2B Antibody Picoband® (A01102-1)
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