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Product Info Summary
| SKU: | M01114 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-CD40L CD40LG Rabbit Monoclonal Antibody
View all CD40 Ligand/TNFSF5 Antibodies
SKU/Catalog Number
M01114
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-CD40L CD40LG Rabbit Monoclonal Antibody catalog # M01114. Tested in WB application. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD40L CD40LG Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01114)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AAIE-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human CD40L
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01114 is reactive to CD40LG in Human, Mouse
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
85 kDa
Calculated molecular weight
29274 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01114 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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Western blot analysis of CD40L expression in MOLT-4 cell lysate.
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
Protein Target Info & Infographic
Gene/Protein Information For CD40LG (Source: Uniprot.org, NCBI)
Gene Name
CD40LG
Full Name
CD40 ligand
Weight
29274 MW
Superfamily
tumor necrosis factor family
Alternative Names
CD40 ligand;CD40-L;T-cell antigen Gp39;TNF-related activation protein;TRAP;Tumor necrosis factor ligand superfamily member 5;CD154;CD40 ligand, membrane form;CD40 ligand, soluble form;CD40LG;CD40L, TNFSF5, TRAP; CD40LG CD154, CD40L, HIGM1, IGM, IMD3, T-BAM, TNFSF5, TRAP, gp39, hCD40L CD40 ligand CD40 ligand|CD40 ligand|CD40-L|T-B cell-activating molecule|T-cell Gp39|TNF-related activation protein|tumor necrosis factor (ligand) superfamily member 5
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CD40LG, check out the CD40LG Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CD40LG: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CD40L CD40LG Rabbit Monoclonal Antibody (M01114)
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No publications found for M01114
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Customer Q&As
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6 Customer Q&As for Anti-CD40L CD40LG Rabbit Monoclonal Antibody
Question
We have seen staining in human blood. Do you have any suggestions? Is anti-CD40L Rabbit Monoclonal antibody supposed to stain blood positively?
Verified Customer
Verified customer
Asked: 2020-02-17
Answer
Based on literature blood does express CD40LG. Based on Uniprot.org, CD40LG is expressed in leukocyte, blood, among other tissues. Regarding which tissues have CD40LG expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2020-02-17
Question
Is this M01114 anti-CD40L Rabbit Monoclonal antibody reactive to the isotypes of CD40LG?
Verified Customer
Verified customer
Asked: 2019-12-27
Answer
The immunogen of M01114 anti-CD40L Rabbit Monoclonal antibody is A synthesized peptide derived from human CD40L. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-12-27
Question
My boss were happy with the WB result of your anti-CD40L Rabbit Monoclonal antibody. However we have observed positive staining in leukocyte cell membrane using this antibody. Is that expected? Could you tell me where is CD40LG supposed to be expressed?
Verified Customer
Verified customer
Asked: 2017-12-05
Answer
Based on literature, leukocyte does express CD40LG. Generally CD40LG expresses in cell membrane. Regarding which tissues have CD40LG expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2017-12-05
Question
I am looking for to test anti-CD40L Rabbit Monoclonal antibody M01114 on mouse blood for research purposes, then I may be interested in using anti-CD40L Rabbit Monoclonal antibody M01114 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2017-07-28
Answer
The products we sell, including anti-CD40L Rabbit Monoclonal antibody M01114, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-07-28
Question
I was wanting to use your anti-CD40L Rabbit Monoclonal antibody for WB for mouse blood on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse blood identification?
Verified Customer
Verified customer
Asked: 2017-06-30
Answer
As indicated on the product datasheet, M01114 anti-CD40L Rabbit Monoclonal antibody has been validated for WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse blood in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-06-30
Question
We are currently using anti-CD40L Rabbit Monoclonal antibody M01114 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it likely that the antibody can work on dog tissues as well?
A. Li
Verified customer
Asked: 2016-10-06
Answer
The anti-CD40L Rabbit Monoclonal antibody (M01114) has not been validated for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-10-06
