Product Info Summary
SKU: | A06184 |
---|---|
Size: | 100μl |
Reactive Species: | Human |
Host: | Rabbit |
Application: | IHC |
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Product info
Product Name
Anti-CD1E Antibody
SKU/Catalog Number
A06184
Size
100μl
Form
Liquid
Description
Boster Bio Anti-CD1E Antibody catalog # A06184. Tested in IHC applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD1E Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06184)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from the C-terminal region of human CD1E. AA range:321-370
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A06184 is reactive to CD1E in Human
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
43626 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A06184 is guaranteed for IHC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
IHC-P, 1:100-300
Validation Images & Assay Conditions
Click image to see more details
Western Blot (WB) analysis of K562 cells using CD1E Polyclonal antibody.
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Immunohistochemistry (IHC) analysis of paraffin-embedded Human Pancreas, antibody was diluted at 1:100.
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Immunohistochemistry (IHC) analysis of paraffin-embedded Human Pancreas, antibody was diluted at 1:100.
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Immunohistochemistry (IHC) analysis of paraffin-embedded Human Pancreas, antibody was diluted at 1:100.
Protein Target Info & Infographic
Gene/Protein Information For CD1E (Source: Uniprot.org, NCBI)
Gene Name
CD1E
Full Name
T-cell surface glycoprotein CD1e, membrane-associated
Weight
43626 MW
Alternative Names
T-cell surface glycoprotein CD1e, membrane-associated;hCD1e;R2G1;CD1e;T-cell surface glycoprotein CD1e, soluble;sCD1e;CD1E; CD1E CD1A, R2 CD1e molecule T-cell surface glycoprotein CD1e, membrane-associated|CD1E , e polypeptide|R2G1|differentiation CD1-alpha-3|hCD1e|leukocyte differentiation |thymocyte CD1E
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CD1E, check out the CD1E Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CD1E: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CD1E Antibody (A06184)
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No publications found for A06184
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Customer Q&As
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4 Customer Q&As for Anti-CD1E Antibody
Question
I have a question about product A06184, anti-CD1E antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-09-02
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A06184 anti-CD1E antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-09-02
Question
Is this A06184 anti-CD1E antibody reactive to the isotypes of CD1E?
V. Miller
Verified customer
Asked: 2019-07-16
Answer
The immunogen of A06184 anti-CD1E antibody is Synthesized peptide derived from the C-terminal region of human CD1E. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-07-16
Question
We are currently using anti-CD1E antibody A06184 for human tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on monkey tissues as well?
V. Zhao
Verified customer
Asked: 2019-05-10
Answer
The anti-CD1E antibody (A06184) has not been tested for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-05-10
Question
See attached the WB image, lot number and protocol we used for thymus using anti-CD1E antibody A06184. Please let me know if you require anything else.
K. Krishna
Verified customer
Asked: 2017-02-08
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-02-08