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Product Info Summary
| SKU: | A02158-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-CD1B Antibody Picoband®
SKU/Catalog Number
A02158-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CD1B Antibody Picoband® catalog # A02158-2. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-CD1B Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02158-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human CD1B recombinant protein (Position: D78-Y110).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A02158-2 is reactive to CD1B in Human, Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
40 kDa
Calculated molecular weight
63692 MW
Background of CD1B
CD1B is a member of the CD1 family of transmembrane glycoproteins, which are structurally related to the major histocompatibility complex (MHC) proteins and form heterodimers with beta-2-microglobulin. The CD1 proteins mediate the presentation of primarily lipid and glycolipid antigens of self or microbial origin to T cells. The human genome contains five CD1 family genes organized in a cluster on chromosome 1. The CD1 family members are thought to differ in their cellular localization and specificity for particular lipid ligands. The protein encoded by this gene localizes to late endosomes and lysosomes via a tyrosine-based motif in the cytoplasmic tail, and requires vesicular acidification to bind lipid antigens.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02158-2 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human HCCT tissue, human MOLT-4 tissue, human Jurkat whole cell, rat thymus tissue, mouse thymus tissue, mouse RAW2647 whole cell, mouse Ana-1 whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of CD1B using anti-CD1B antibody (A02158-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HCCT tissue lysates,
Lane 2: human MOLT-4 tissue lysates,
Lane 3: human Jurkat whole cell lysates,
Lane 4: rat thymus tissue lysates,
Lane 5: mouse thymus tissue lysates,
Lane 6: mouse RAW264.7 whole cell lysates,
Lane 7: mouse Ana-1 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD1B antigen affinity purified polyclonal antibody (Catalog # A02158-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD1B at approximately 40 kDa. The expected band size for CD1B is at 37 kDa.
Protein Target Info & Infographic
Gene/Protein Information For CD1B (Source: Uniprot.org, NCBI)
Gene Name
CD1B
Full Name
T-cell surface glycoprotein CD1b
Weight
63692 MW
Alternative Names
T-cell surface glycoprotein CD1b; CD1b; CD1B CD1B CD1, CD1A, R1 CD1b molecule T-cell surface glycoprotein CD1b|CD1B , b polypeptide|cortical thymocyte CD1B|differentiation CD1-alpha-3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CD1B, check out the CD1B Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CD1B: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CD1B Antibody Picoband® (A02158-2)
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