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Product Info Summary
| SKU: | M00375 |
|---|---|
| Size: | 100ug/vial |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | Flow Cytometry, IF, IHC |
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Product info
Product Name
Anti-CD1a / HTA1 (Mature Langerhans Cells Marker) Monoclonal Antibody
SKU/Catalog Number
M00375
Size
100ug/vial
Form
Liquid
Description
Boster Bio Anti-CD1a / HTA1 (Mature Langerhans Cells Marker) Monoclonal Antibody (Catalog # M00375). Tested in Flow Cytometry, IF, IHC applications. This antibody reacts with Human.
Storage & Handling
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Cite This Product
Anti-CD1a / HTA1 (Mature Langerhans Cells Marker) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00375)
Host
Mouse
Contents
Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Clonality
Monoclonal
Clone Number
Clone: O10 + C1A/711
Isotype
IgG1, kappa + IgG1, kappa
Immunogen
Human thymus cells (O10); Recombinant human CD1a protein (C1A/711)
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00375 is reactive to CD1A in Human
Reconstitution
Reconstitute with 1ml water (15 minutes at room temperature).
Calculated molecular weight
37077 MW
Background of CD1A
At least five CD1 genes (CD1a, b, c, d, and e) are identified. CD1 proteins have been demonstrated to restrict T cell response to non-peptide lipid and glycolipid antigens and play a role in non-classical antigen presentation. CD1a is a non-polymorphic MHC Class 1 related cell surface glycoprotein, expressed in association with Beta-2 microglobulin. Anti-CD1a labels Langerhans cell histiocytosis (Histiocytosis X), extranodal histiocytic sarcoma, a subset of T-lymphoblastic lymphoma/leukemia, and interdigitating dendritic cell sarcoma of the lymph node. When combined with antibodies against TTF-1 and CD5, anti-CD1a is useful in distinguishing between pulmonary and thymic neoplasms since CD1a is consistently expressed in thymic lymphocytes in both typical and atypical thymomas, but only focally in 1/6 of thymic carcinomas and not in lymphocytes in pulmonary neoplasms. Anti-CD1a is reported to be a new marker for perivascular epithelial cell tumor (PEComa).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00375 is guaranteed for Flow Cytometry, IF, IHC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.
Validation Images & Assay Conditions
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Formalin-fixed, paraffin-embedded human Skin stained with Anti-CD1a Monoclonal Antibody (O10+C1A/711).
Protein Target Info & Infographic
Gene/Protein Information For CD1A (Source: Uniprot.org, NCBI)
Gene Name
CD1A
Full Name
T-cell surface glycoprotein CD1a
Weight
37077 MW
Alternative Names
T-cell surface glycoprotein CD1a;T-cell surface antigen T6/Leu-6;hTa1 thymocyte antigen;CD1a;CD1A; CD1A CD1, FCB6, HTA1, R4, T6 CD1a molecule T-cell surface glycoprotein CD1a|CD1A , a polypeptide|T-cell surface T6/Leu-6|cluster of differentiation 1 A|cortical thymocyte CD1A|differentiation CD1-alpha-3|epidermal dendritic cell marker CD1a|hTa1 thymocyte
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CD1A, check out the CD1A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CD1A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CD1a / HTA1 (Mature Langerhans Cells Marker) Monoclonal Antibody (M00375)
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No publications found for M00375
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Customer Q&As
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3 Customer Q&As for Anti-CD1a / HTA1 (Mature Langerhans Cells Marker) Monoclonal Antibody
Question
We are currently using anti-CD1a / HTA1 (Mature Langerhans Cells Marker) Monoclonal antibody M00375 for human tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2020-03-06
Answer
The anti-CD1a / HTA1 (Mature Langerhans Cells Marker) Monoclonal antibody (M00375) has not been tested for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-03-06
Question
My colleagues were satisfied with the WB result of your anti-CD1a / HTA1 (Mature Langerhans Cells Marker) Monoclonal antibody. However we have seen positive staining in thymus cell membrane using this antibody. Is that expected? Could you tell me where is CD1A supposed to be expressed?
Verified Customer
Verified customer
Asked: 2018-05-08
Answer
Based on literature, thymus does express CD1A. Generally CD1A expresses in cell membrane. Regarding which tissues have CD1A expression, here are a few articles citing expression in various tissues:
T-cell, Pubmed ID: 3093894
Thymus, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2018-05-08
Question
We have seen staining in human thymus. Do you have any suggestions? Is anti-CD1a / HTA1 (Mature Langerhans Cells Marker) Monoclonal antibody supposed to stain thymus positively?
A. Brown
Verified customer
Asked: 2017-03-09
Answer
Based on literature thymus does express CD1A. Based on Uniprot.org, CD1A is expressed in thymus, t-cell, among other tissues. Regarding which tissues have CD1A expression, here are a few articles citing expression in various tissues:
T-cell, Pubmed ID: 3093894
Thymus, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2017-03-09
