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Product Info Summary
| SKU: | PA1875 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-CD1a Antibody Picoband®
SKU/Catalog Number
PA1875
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CD1a Antibody catalog # PA1875. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD1a Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1875)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human CD1a.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1875 is reactive to CD1A in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
37 kDa
Calculated molecular weight
37077 MW
Background of CD1A
CD1A (Thymocyte antigen CD1A) also known as CD1, is a human protein encoded by the CD1A gene. CD1A is 1 of 5 distinct CD1 genes that are variably conserved in different mammalian species. This gene encodes a member of the CD1 family of transmembrane glycoproteins, which are structurally related to the major histocompatibility complex (MHC) proteins and form heterodimers with beta-2-microglobulin.The CD1A gene is mapped on 1q23.1. The CD1 proteins mediate the presentation of primarily lipid and glycolipid antigens of self or microbial origin to T cells. CD1A is concentrated in the early or recycling endosome. Moody et al. (2004) reported that CD1A presents to T cells a family of previously unknown lipopeptides from Mycobacterium tuberculosis, named didehydroxymycobactins because of their structural relation to mycobactin siderophores. The protein encoded by this gene localizes to the plasma membrane and to recycling vesicles of the early endocytic system. Apolipoprotein E (107741) binds lipid antigens and delivers them by receptor-mediated uptake into endosomal compartments containing CD1 in antigen-presenting cells.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1875 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: human Jurkat whole cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of CD1a using anti-CD1a antibody (PA1875).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Jurkat whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD1a antigen affinity purified polyclonal antibody (Catalog # PA1875) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD1a at approximately 37 kDa. The expected band size for CD1a is at 37 kDa.
Protein Target Info & Infographic
Gene/Protein Information For CD1A (Source: Uniprot.org, NCBI)
Gene Name
CD1A
Full Name
T-cell surface glycoprotein CD1a
Weight
37077 MW
Alternative Names
T-cell surface glycoprotein CD1a;T-cell surface antigen T6/Leu-6;hTa1 thymocyte antigen;CD1a;CD1A; CD1A CD1, FCB6, HTA1, R4, T6 CD1a molecule T-cell surface glycoprotein CD1a|CD1A , a polypeptide|T-cell surface T6/Leu-6|cluster of differentiation 1 A|cortical thymocyte CD1A|differentiation CD1-alpha-3|epidermal dendritic cell marker CD1a|hTa1 thymocyte
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CD1A, check out the CD1A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CD1A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CD1a Antibody Picoband® (PA1875)
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Customer Q&As
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16 Customer Q&As for Anti-CD1a Antibody Picoband®
Question
I see that the anti-CD1a antibody PA1875 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-04-10
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-04-10
Question
I was wanting to use your anti-CD1a antibody for WB for human t-cell on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human t-cell identification?
Verified Customer
Verified customer
Asked: 2020-01-20
Answer
It shows on the product datasheet, PA1875 anti-CD1a antibody has been validated for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human t-cell in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-01-20
Question
We want to test anti-CD1a antibody PA1875 on human t-cell for research purposes, then I may be interested in using anti-CD1a antibody PA1875 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
K. Bhatt
Verified customer
Asked: 2019-10-10
Answer
The products we sell, including anti-CD1a antibody PA1875, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-10-10
Question
Is there a BSA free version of anti-CD1a antibody PA1875 available?
Verified Customer
Verified customer
Asked: 2019-06-21
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-CD1a antibody PA1875 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-06-21
Question
We are currently using anti-CD1a antibody PA1875 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2019-01-02
Answer
The anti-CD1a antibody (PA1875) has not been tested for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-01-02
Question
We have seen staining in human t-cell. What should we do? Is anti-CD1a antibody supposed to stain t-cell positively?
Verified Customer
Verified customer
Asked: 2018-10-31
Answer
According to literature t-cell does express CD1A. According to Uniprot.org, CD1A is expressed in thymus, t-cell, among other tissues. Regarding which tissues have CD1A expression, here are a few articles citing expression in various tissues:
T-cell, Pubmed ID: 3093894
Thymus, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2018-10-31
Question
Our team were content with the WB result of your anti-CD1a antibody. However we have seen positive staining in thymus cell membrane using this antibody. Is that expected? Could you tell me where is CD1A supposed to be expressed?
Verified Customer
Verified customer
Asked: 2018-04-02
Answer
According to literature, thymus does express CD1A. Generally CD1A expresses in cell membrane. Regarding which tissues have CD1A expression, here are a few articles citing expression in various tissues:
T-cell, Pubmed ID: 3093894
Thymus, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2018-04-02
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for t-cell using anti-CD1a antibody PA1875. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2017-12-22
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-12-22
Question
Does anti-CD1a antibody PA1875 work on goat WB with t-cell?
Verified Customer
Verified customer
Asked: 2017-11-08
Answer
Our lab technicians have not tested anti-CD1a antibody PA1875 on goat. You can run a BLAST between goat and the immunogen sequence of anti-CD1a antibody PA1875 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat t-cell in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-11-08
Question
Would PA1875 anti-CD1a antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2017-10-31
Answer
It shows on the product datasheet, PA1875 anti-CD1a antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-10-31
Question
Is a blocking peptide available for product anti-CD1a antibody (PA1875)?
Verified Customer
Verified customer
Asked: 2017-09-29
Answer
We do provide the blocking peptide for product anti-CD1a antibody (PA1875). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2017-09-29
Question
See attached the WB image, lot number and protocol we used for t-cell using anti-CD1a antibody PA1875. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2017-06-30
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-06-30
Question
Will anti-CD1a antibody PA1875 work for WB with t-cell?
C. Kulkarni
Verified customer
Asked: 2015-11-09
Answer
According to the expression profile of t-cell, CD1A is highly expressed in t-cell. So, it is likely that anti-CD1a antibody PA1875 will work for WB with t-cell.
Boster Scientific Support
Answered: 2015-11-09
Question
We need using your anti-CD1a antibody for adaptive immune response studies. Has this antibody been tested with western blotting on cem whole cell lysate? We would like to see some validation images before ordering.
D. Zhang
Verified customer
Asked: 2015-10-22
Answer
We appreciate your inquiry. This PA1875 anti-CD1a antibody is tested on jurkat whole cell lysate, sw620 whole cell lysate, cem whole cell lysate. It is guaranteed to work for WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2015-10-22
Question
Can you help my question with product PA1875, anti-CD1a antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
K. Singh
Verified customer
Asked: 2015-04-27
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1875 anti-CD1a antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2015-04-27
Question
Is this PA1875 anti-CD1a antibody reactive to the isotypes of CD1A?
E. Parker
Verified customer
Asked: 2014-09-15
Answer
The immunogen of PA1875 anti-CD1a antibody is A synthetic peptide corresponding to a sequence in the middle region of human CD1a(158-173aa AKHFCKVLNQNQHEND). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2014-09-15
