Anti-CD162 (Selectin P Ligand) SELPLG Monoclonal Antibody

Product Name

Anti-CD162 (Selectin P Ligand) SELPLG Monoclonal Antibody

View all PSGL-1/CD162 Antibodies

SKU/Catalog Number

M03674

Size

100ug/vial

Form

Liquid

Description

Boster Bio Anti-CD162 (Selectin P Ligand) SELPLG Monoclonal Antibody (Catalog # M03674). Tested in IHC applications. This antibody reacts with Human.

Storage & Handling

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Cite This Product

Anti-CD162 (Selectin P Ligand) SELPLG Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03674)

Host

Mouse

Contents

Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Clonality

Monoclonal

Clone Number

Clone: PSGL1/1601

Isotype

IgG1, kappa

Immunogen

Recombinant human SELPLG (CD162) protein

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Does not cross-react with primate, avian or amphibian GR.

Reactive Species

M03674 is reactive to SELPLG in Human

Reconstitution

Reconstitute with distilled water.

Observed Molecular Weight

68 kDa

Calculated molecular weight

43201 MW

Background of PSGL-1/CD162

CD162 glycoprotein functions as a high affinity counter-receptor for the cell adhesion molecules P-, E- and L- selectin expressed on myeloid cells and stimulated T lymphocytes. As such, this protein plays a critical role in leukocyte trafficking during inflammation by tethering of leukocytes to activated platelets or endothelia expressing selectins. This protein requires two post-translational modifications, tyrosine sulfation and the addition of the sialyl Lewis x tetrasaccharide (sLex) to its O-linked glycans, for its high-affinity binding activity. Aberrant expression of this gene and polymorphisms in this gene are associated with defects in the innate and adaptive immune response.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M03674 is guaranteed for IHC Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Validation Images & Assay Conditions

Gene/Protein Information For SELPLG (Source: Uniprot.org, NCBI)

Alternative Names

P-selectin glycoprotein ligand 1;PSGL-1;Selectin P ligand;CD162;SELPLG; SELPLG CD162, CLA, PSGL-1, PSGL1 selectin P ligand P-selectin glycoprotein ligand 1|cutaneous lymphocyte-associated associated

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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