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Product Info Summary
| SKU: | A02512-1 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-CD100 Antibody
View all Semaphorin 4D/CD100 Antibodies
SKU/Catalog Number
A02512-1
Size
100μl
Form
Liquid
Description
Boster Bio Anti-CD100 Antibody catalog # A02512-1. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD100 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02512-1)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from the N-terminal region of human SEMA4D. AA range:61-110
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A02512-1 is reactive to SEMA4D in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
96150 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02512-1 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
Validation Images & Assay Conditions
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Western Blot (WB) analysis of MCF7, L929 cells using CD100 Polyclonal antibody.
Protein Target Info & Infographic
Gene/Protein Information For SEMA4D (Source: Uniprot.org, NCBI)
Gene Name
SEMA4D
Full Name
Semaphorin-4D
Weight
96150 MW
Superfamily
semaphorin family
Alternative Names
Semaphorin-4D;A8;BB18;GR3;CD100;SEMA4D;C9orf164, CD100, SEMAJ; SEMA4D A8, BB18, C9orf164, CD100, COLL4, GR3, M-sema-G, SEMAJ, coll-4 semaphorin 4D semaphorin-4D|sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D|sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, 4D
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SEMA4D, check out the SEMA4D Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SEMA4D: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CD100 Antibody (A02512-1)
Hello CJ!
A02512-1 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Semaphorin 4D and hypoxia-inducible factor-1α overexpression is related to prognosis in colorectal carcinoma
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-CD100 Antibody
Question
My question regards to test anti-CD100 antibody A02512-1 on mouse c1 segment of cervical spinal cord for research purposes, then I may be interested in using anti-CD100 antibody A02512-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-03-27
Answer
The products we sell, including anti-CD100 antibody A02512-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-03-27
Question
Is a blocking peptide available for product anti-CD100 antibody (A02512-1)?
Verified Customer
Verified customer
Asked: 2020-01-10
Answer
We do provide the blocking peptide for product anti-CD100 antibody (A02512-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-01-10
Question
Please see the WB image, lot number and protocol we used for c1 segment of cervical spinal cord using anti-CD100 antibody A02512-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-10-31
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-31
Question
I was wanting to use your anti-CD100 antibody for WB for mouse c1 segment of cervical spinal cord on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse c1 segment of cervical spinal cord identification?
Verified Customer
Verified customer
Asked: 2019-08-20
Answer
You can see on the product datasheet, A02512-1 anti-CD100 antibody has been tested for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse c1 segment of cervical spinal cord in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-08-20
Question
Is this A02512-1 anti-CD100 antibody reactive to the isotypes of SEMA4D?
Verified Customer
Verified customer
Asked: 2017-12-11
Answer
The immunogen of A02512-1 anti-CD100 antibody is Synthesized peptide derived from the N-terminal region of human CD100. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-12-11
Question
We are currently using anti-CD100 antibody A02512-1 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on dog tissues as well?
A. Zhang
Verified customer
Asked: 2017-01-18
Answer
The anti-CD100 antibody (A02512-1) has not been validated for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-01-18
