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Product Info Summary
| SKU: | A01896-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-CCR1 Antibody Picoband®
SKU/Catalog Number
A01896-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CCR1 Antibody Picoband® catalog # A01896-2. Tested in WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-CCR1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01896-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of mouse CCR1, which shares 73.7% amino acid (aa) sequence identity with human CCR1, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A01896-2 is reactive to CCR1 in Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
41 kDa
Calculated molecular weight
41 kDa
Background of CCR1
C-C chemokine receptor type 1 is a protein that in humans is encoded by the CCR1 gene. This gene encodes a member of the beta chemokine receptor family, which is predicted to be a seven transmembrane protein similar to G protein-coupled receptors. The ligands of this receptor include macrophage inflammatory protein 1 alpha (MIP-1 alpha), regulated on activation normal T expressed and secreted protein (RANTES), monocyte chemoattractant protein 3 (MCP-3), and myeloid progenitor inhibitory factor-1 (MPIF-1). Chemokines and their receptors mediated signal transduction are critical for the recruitment of effector immune cells to the site of inflammation. Knockout studies of the mouse homolog suggested the roles of this gene in host protection from inflammatory response, and susceptibility to virus and parasite. This gene and other chemokine receptor genes, including CCR2, CCRL2, CCR3, CCR5 and CCXCR1, are found to form a gene cluster on chromosome 3p.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01896-2 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Mouse, Rat
Positive Control
WB: rat heart tissue, mouse heart tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of CCR1 using anti-CCR1 antibody (A01896-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat heart tissue lysates,
Lane 2: mouse heart tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCR1 antigen affinity purified polyclonal antibody (Catalog # A01896-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CCR1 at approximatel 41 kDa. The expected band size for CCR1 is at 41 kDa.
Protein Target Info & Infographic
Gene/Protein Information For CCR1 (Source: Uniprot.org, NCBI)
Gene Name
CCR1
Full Name
C-C chemokine receptor type 1
Weight
41 kDa
Superfamily
G-protein coupled receptor 1 family
Alternative Names
C-C chemokine receptor type 1; CC-CKR-1; CCR1; CCR-1; CD191 antigen; CD191; chemokine (C-C motif) receptor 1; CKR1; CKR-1; CMKBR1SCYAR1; CMKR1; HM145C-C CKR-1; LD78 receptor; Macrophage inflammatory protein 1-alpha receptor; MIP-1alpha-R; MIP1aR; RANTES receptor; RANTES-R CCR1 CD191, CKR-1, CKR1, CMKBR1, HM145, MIP1aR, SCYAR1 C-C motif chemokine receptor 1 C-C chemokine receptor type 1|C-C CKR-1|CC-CKR-1|CCR-1|LD78 receptor|MIP-1alpha-R|RANTES receptor|RANTES-R|chemokine (C-C motif) receptor 1|macrophage inflammatory protein 1-alpha receptor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CCR1, check out the CCR1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CCR1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CCR1 Antibody Picoband® (A01896-2)
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