Product Info Summary
SKU: | A13807-1 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human |
Host: | Rabbit |
Application: | ELISA, Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-CCDC115 Antibody Picoband™
SKU/Catalog Number
A13807-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CCDC115 Antibody Picoband™ catalog # A13807-1. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-CCDC115 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A13807-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human CCDC115 recombinant protein (Position: M1-A180).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
A13807-1 is reactive to Ccdc115 in Human
Applications
A13807-1 is guaranteed for ELISA, Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
20 kDa
Calculated molecular weight
64099 MW
Background of Ccdc115
Coiled-coil domain containing 115 is a protein that in humans is encoded by the CCDC115 gene. The protein encoded by this gene has been observed to localize to the endoplasmic reticulum (ER)-Golgi intermediate compartment (ERGIC) and coat protein complex I (COPI) vesicles in some human cells. The encoded protein shares some homology with the yeast V-ATPase assembly factor Vma22p, and the orthologous protein in mouse promotes cell proliferation and suppresses cell death. Defects in this gene are a cause of congenital disorder of glycosylation, type IIo in humans.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
Direct ELISA, 0.1-0.5 μg/ml, Human
Validation Images & Assay Conditions
![a13807 1 ccdc115 primary antibodies wb testing 1 a13807 1 ccdc115 primary antibodies wb testing 1](https://www.bosterbio.com/media/catalog/product/a/1/a13807-1-ccdc115-primary-antibodies-wb-testing-1.jpg)
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Figure 1. Western blot analysis of CCDC115 using anti-CCDC115 antibody (A13807-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: human MOLT-4 whole cell lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: human PC-3 whole cell lysates,
Lane 5: human Daudi whole cell lysates,
Lane 6: human T-47D whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCDC115 antigen affinity purified polyclonal antibody (Catalog # A13807-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CCDC115 at approximately 20 kDa. The expected band size for CCDC115 is at 20 kDa.
![a13807 1 ccdc115 primary antibodies ihc testing 2 a13807 1 ccdc115 primary antibodies ihc testing 2](https://www.bosterbio.com/media/catalog/product/a/1/a13807-1-ccdc115-primary-antibodies-ihc-testing-2.jpg)
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Figure 2. IHC analysis of CCDC115 using anti-CCDC115 antibody (A13807-1).
CCDC115 was detected in a paraffin-embedded section of human laryngeal squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CCDC115 Antibody (A13807-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
![a13807 1 ccdc115 primary antibodies ihc testing 3 a13807 1 ccdc115 primary antibodies ihc testing 3](https://www.bosterbio.com/media/catalog/product/a/1/a13807-1-ccdc115-primary-antibodies-ihc-testing-3.jpg)
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Figure 3. IHC analysis of CCDC115 using anti-CCDC115 antibody (A13807-1).
CCDC115 was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CCDC115 Antibody (A13807-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
![a13807 1 ccdc115 primary antibodies ihc testing 4 a13807 1 ccdc115 primary antibodies ihc testing 4](https://www.bosterbio.com/media/catalog/product/a/1/a13807-1-ccdc115-primary-antibodies-ihc-testing-4.jpg)
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Figure 4. IF analysis of CCDC115 using anti-CCDC115 antibody (A13807-1).
CCDC115 was detected in an immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-CCDC115 Antibody (A13807-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
![a13807 1 ccdc115 primary antibodies fcm testing 5 a13807 1 ccdc115 primary antibodies fcm testing 5](https://www.bosterbio.com/media/catalog/product/a/1/a13807-1-ccdc115-primary-antibodies-fcm-testing-5.jpg)
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Figure 5. Flow Cytometry analysis of U937 cells using anti-CCDC115 antibody (A13807-1).
Overlay histogram showing U937 cells stained with A13807-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CCDC115 Antibody (A13807-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For Ccdc115 (Source: Uniprot.org, NCBI)
Gene Name
Ccdc115
Full Name
Coiled-coil domain-containing protein 115
Weight
64099 MW
Alternative Names
ccp1; coiled-coil domain containing 115; coiled-coil domain-containing protein 115; FLJ30131; MGC12981
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Ccdc115, check out the Ccdc115 Infographic
![Ccdc115 infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Ccdc115: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CCDC115 Antibody Picoband™ (A13807-1)
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