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Product Info Summary
| SKU: | M00152 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | ELISA, Flow Cytometry, IF, IHC, WB |
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Product info
Product Name
Anti-Cbl Monoclonal Antibody
SKU/Catalog Number
M00152
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Cbl Monoclonal Antibody catalog # M00152. Tested in ELISA, Flow Cytometry, IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Cbl Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00152)
Host
Mouse
Contents
Ascitic fluid containing 0.03% sodium azide.
Clonality
Monoclonal
Clone Number
3B12
Isotype
IgG
Immunogen
Purified recombinant fragment of human Cbl expressed in E. Coli.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00152 is reactive to CBL in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
99633 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00152 is guaranteed for ELISA, Flow Cytometry, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:200-1:1000
IF 1:200-1:1000
FC 1:200-1:400
ELISA 1:10000
Validation Images & Assay Conditions
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Figure 5. ELISA validation of CBL using Anti-Cbl Monoclonal Antibody (M00152).
ELISA analysis of Cbl antibody.
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Figure 4. Flow Cytometry validation of CBL using Anti-Cbl Monoclonal Antibody (M00152).
Flow cytometric (FCM) analysis of MCF-7 cells using Cbl Monoclonal Antibody (blue) and negative control (red).
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Figure 3. Immunofluorescent staining data of CBL using Anti-Cbl Monoclonal Antibody (M00152).
Immunofluorescence (IF) analysis of HeLa cells using Cbl Monoclonal Antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
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Figure 2. Immunohistochemistry validation of CBL using Anti-Cbl Monoclonal Antibody (M00152).
Immunohistochemistry (IHC) analysis of paraffin-embedded ovarian cancer tissues with DAB staining using Cbl Monoclonal Antibody.For more protocol information of IHC
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Figure 1. Western blotting validation for Anti-Cbl Monoclonal Antibody M00152
Western Blot (WB) analysis using Cbl Monoclonal Antibody against RAJI (1)Protein Target Info & Infographic
Gene/Protein Information For CBL (Source: Uniprot.org, NCBI)
Gene Name
CBL
Full Name
E3 ubiquitin-protein ligase CBL
Weight
99633 MW
Alternative Names
E3 ubiquitin-protein ligase CBL;2.3.2.27 CBL C-CBL2, FRA11B, NSLL, RNF55, CBL Cbl proto-oncogene E3 ubiquitin-protein ligase CBL|Cas-Br-M (murine) ecotropic retroviral transforming sequence|Cbl proto-oncogene, E3 ubiquitin protein ligase|RING finger protein 55|RING-type E3 ubiquitin transferase CBL|casitas B-lineage lymphoma proto-oncogene|fragile site, folic acid type, rare, fra(11)(q23.3)|oncogene CBL2|proto-oncogene c-Cbl|signal transduction protein CBL
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CBL, check out the CBL Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CBL: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Cbl Monoclonal Antibody (M00152)
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No publications found for M00152
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