Anti-Caspase-3(p17)/CASP3 Antibody Picoband®

Caspase-3 antibody

Boster Bio Anti-Caspase-3(p17)/CASP3 Antibody Picoband® catalog # A00334-2. Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance. Cited in 1 publication(s).

Product Info Summary

SKU: A00334-2
Size: 100 μg/vial
Reactive Species: Human
Host: Rabbit
Application: Flow Cytometry, IF, ICC, WB

Product Name

Anti-Caspase-3(p17)/CASP3 Antibody Picoband®

View all Caspase-3 Antibodies

SKU/Catalog Number

A00334-2

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Caspase-3(p17)/CASP3 Antibody Picoband® catalog # A00334-2. Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-Caspase-3(p17)/CASP3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00334-2)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human Caspase-3(p17)/CASP3, which shares 78.3% and 87% amino acid (aa) sequence identity with mouse and rat CASP3.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A00334-2 is reactive to CASP3 in Human

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Observed Molecular Weight

32 kDa

Calculated molecular weight

133277 MW

Background of Caspase-3

Caspase 3 is a caspase protein which interacts with Survivin, XIAP, CFLAR, Caspase 8, HCLS1, Deleted in Colorectal Cancer, TRAF3 and GroEL. This gene which is located on 4q35 encodes a protein that is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes that undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. And the caspase-3 activation in heart failure sequentially cleaves SRF and generates a truncated SRF that appears to function as a dominant-negative transcription factor. Additionally, the caspase-3 influence on bone mineral absorbance should be considered in any in vivo application of caspase-3 inhibitors to the treatment of human disease. In erythroid precursors undergoing terminal differentiation, Hsp70 prevents active CASP3 from cleaving GATA1 and inducing apoptosis.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A00334-2 is guaranteed for Flow Cytometry, IF, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human

Positive Control

WB: human Jurkat whole cell, human Hela whole cell, human 293T whole cell, human HepG2 whole cell
ICC/IF: SiHa cell
FCM: Caco-2 cell

Validation Images & Assay Conditions

Gene/Protein Information For CASP3 (Source: Uniprot.org, NCBI)

Gene Name

CASP3

Full Name

Caspase-3

Weight

133277 MW

Superfamily

peptidase C14A family

Alternative Names

Menin; MEN1; SCG2 CASP3 CPP32, CPP32B, SCA-1 caspase 3 caspase-3|CASP-3|CPP-32|PARP cleavage protease|SREBP cleavage activity 1|apopain|caspase 3, apoptosis-related cysteine peptidase|caspase 3, apoptosis-related cysteine protease|cysteine protease CPP32|procaspase3|protein Yama

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on CASP3, check out the CASP3 Infographic

CASP3 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for CASP3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

A00334-2 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

High Expression of Pirh2 is Associated with Poor Prognosis in Glioma

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Order DetailsPrice
A00334-2

100μg

$370
A00334-2-10ug

10μg sample (liquid)

$99
A00334-2-Biotin

100 μg Biotin conjugated

$570
A00334-2-Cy3

100 μg Cy3 conjugated

$570
A00334-2-Dylight488

100 μg Dylight488 conjugated

$570
A00334-2-Dylight550

100 μg Dylight550 conjugated

$570
A00334-2-Dylight594

100 μg Dylight594 conjugated

$570
A00334-2-FITC

100 μg FITC conjugated

$570
A00334-2-HRP

100 μg HRP conjugated

$570
A00334-2-APC

100 μg APC conjugated

$670
A00334-2-PE

100 μg PE conjugated

$670
A00334-2-iFluor647

100 μg iFluor647 conjugated

$670
A00334-2-carrier-free

Carrier Free

$370

More conjugates/formats

Free Secondary Antibody

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A00334-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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