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Product Info Summary
| SKU: | A11515 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC-P, WB, IF (paraffin) |
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Product info
Product Name
Anti-CaMK1-beta PNCK Antibody
SKU/Catalog Number
A11515
Size
100ul
Form
Liquid
Description
Boster Bio Anti-CaMK1-beta PNCK Antibody (Catalog# A11515). Tested in WB, IHC-P, IF (paraffin), ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CaMK1-beta PNCK Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A11515)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human CaMK1-beta. Immunogen sequence location: 161-210
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A11515 is reactive to PNCK in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
72 kDa
Calculated molecular weight
38500 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A11515 is guaranteed for ELISA, IHC-P, WB, IF (paraffin) Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western Blot, 1:500 - 1:2000
Immunohistochemistry, 1:100 - 1:300
ELISA, 1:10000
Not yet tested in other applications.
Validation Images & Assay Conditions
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Western blot analysis of lysates from LOVO cells, treated with H2O2 100uM 30', using CaMK1-beta Antibody. The lane on the right is blocked with the synthesized peptide.
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Immunohistochemistry analysis of paraffin-embedded human brain tissue, using CaMK1-beta Antibody. The picture on the right is blocked with the synthesized peptide.
Protein Target Info & Infographic
Gene/Protein Information For PNCK (Source: Uniprot.org, NCBI)
Gene Name
PNCK
Full Name
Calcium/calmodulin-dependent protein kinase type 1B
Weight
38500 MW
Superfamily
protein kinase superfamily
Alternative Names
PNCK;Calcium/calmodulin-dependent protein kinase type 1B;CaM kinase I beta;CaM kinase IB;CaM-KI beta;CaMKI-beta;Pregnancy up-regulated non-ubiquitously-expressed CaM kinase Pnck|Camk1b|pregnancy up-regulated nonubiquitous CaM kinase|calcium/calmodulin-dependent protein kinase type 1B|Protein Kinase-like|caM kinase I beta|caM kinase IB|caM-KI beta|caMKI-beta|calcium/calmodulin-dependent protein kinase 1, beta|pregnancy up-regulated non-ubiquitously-expressed CaM kinase homolog|pregnancy upregulated non-ubiquitously expressed CaM kinase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PNCK, check out the PNCK Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PNCK: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CaMK1-beta PNCK Antibody (A11515)
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No publications found for A11515
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Customer Q&As
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6 Customer Q&As for Anti-CaMK1-beta PNCK Antibody
Question
We are currently using anti-CaMK1-beta antibody A11515 for rat tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2018-09-10
Answer
The anti-CaMK1-beta antibody (A11515) has not been tested for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-09-10
Question
I see that the anti-CaMK1-beta antibody A11515 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-06-05
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-06-05
Question
Does anti-CaMK1-beta antibody A11515 work on horse IHC with brain?
G. Anderson
Verified customer
Asked: 2018-04-24
Answer
Our lab technicians have not validated anti-CaMK1-beta antibody A11515 on horse. You can run a BLAST between horse and the immunogen sequence of anti-CaMK1-beta antibody A11515 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse brain in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-04-24
Question
Does A11515 anti-CaMK1-beta antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
C. Walker
Verified customer
Asked: 2017-11-20
Answer
As indicated on the product datasheet, A11515 anti-CaMK1-beta antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-11-20
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver thalamus using anti-CaMK1-beta antibody A11515. Let me know if you need anything else.
N. Evans
Verified customer
Asked: 2016-11-10
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2016-11-10
Question
Can you help my question with product A11515, anti-CaMK1-beta antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
S. Huang
Verified customer
Asked: 2016-10-11
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A11515 anti-CaMK1-beta antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2016-10-11
