Anti-CaMK1-beta PNCK Antibody

Pnck antibody

Boster Bio Anti-CaMK1-beta PNCK Antibody (Catalog# A11515). Tested in WB, IHC-P, IF (paraffin), ELISA applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: A11515
Size: 100ul
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: ELISA, IHC-P, WB, IF (paraffin)

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Product Name

Anti-CaMK1-beta PNCK Antibody

View all Pnck Antibodies

SKU/Catalog Number

A11515

Size

100ul

Form

Liquid

Description

Boster Bio Anti-CaMK1-beta PNCK Antibody (Catalog# A11515). Tested in WB, IHC-P, IF (paraffin), ELISA applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-CaMK1-beta PNCK Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A11515)

Host

Rabbit

Contents

PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A peptide derived from human CaMK1-beta. Immunogen sequence location: 161-210

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A11515 is reactive to Pnck in Human, Mouse, Rat

Applications

A11515 is guaranteed for ELISA, IHC-P, WB, IF (paraffin) Boster Guarantee

Observed Molecular Weight

72 kDa

Calculated molecular weight

38500 MW

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western Blot, 1:500 - 1:2000
Immunohistochemistry, 1:100 - 1:300
ELISA, 1:10000
Not yet tested in other applications.

Validation Images & Assay Conditions

Gene/Protein Information For Pnck (Source: Uniprot.org, NCBI)

Gene Name

Pnck

Full Name

Calcium/calmodulin-dependent protein kinase type 1B

Weight

38500 MW

Superfamily

protein kinase superfamily

Alternative Names

BSTK3; calcium/calmodulin-dependent protein kinase type 1B; CaM kinase I beta; CaM kinase IB; CaMK1b; CaM-KI beta; caMKI-beta; EC 2.7.11; EC 2.7.11.17; FLJ50403; FLJ50549; FLJ56451; FLJ59811; MGC45419; pregnancy up-regulated non-ubiquitously expressed CaM kinase; pregnancy upregulated non-ubiquitously expressed CaM kinase; Pregnancy up-regulated non-ubiquitously-expressed CaM kinase Pnck|Camk1b|pregnancy up-regulated nonubiquitous CaM kinase|calcium/calmodulin-dependent protein kinase type 1B|Protein Kinase-like|caM kinase I beta|caM kinase IB|caM-KI beta|caMKI-beta|calcium/calmodulin-dependent protein kinase 1, beta|pregnancy up-regulated non-ubiquitously-expressed CaM kinase homolog|pregnancy upregulated non-ubiquitously expressed CaM kinase

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on Pnck, check out the Pnck Infographic

Pnck infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for Pnck: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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6 Customer Q&As for Anti-CaMK1-beta PNCK Antibody

Question

We are currently using anti-CaMK1-beta antibody A11515 for rat tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on bovine tissues as well?

Verified Customer

Verified customer

Asked: 2018-09-10

Answer

The anti-CaMK1-beta antibody (A11515) has not been tested for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2018-09-10

Question

I see that the anti-CaMK1-beta antibody A11515 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2018-06-05

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2018-06-05

Question

Does anti-CaMK1-beta antibody A11515 work on horse IHC with brain?

G. Anderson

Verified customer

Asked: 2018-04-24

Answer

Our lab technicians have not validated anti-CaMK1-beta antibody A11515 on horse. You can run a BLAST between horse and the immunogen sequence of anti-CaMK1-beta antibody A11515 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse brain in IHC, you can get your next antibody for free.

Boster Scientific Support

Answered: 2018-04-24

Question

Does A11515 anti-CaMK1-beta antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

C. Walker

Verified customer

Asked: 2017-11-20

Answer

As indicated on the product datasheet, A11515 anti-CaMK1-beta antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2017-11-20

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver thalamus using anti-CaMK1-beta antibody A11515. Let me know if you need anything else.

N. Evans

Verified customer

Asked: 2016-11-10

Answer

Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2016-11-10

Question

Can you help my question with product A11515, anti-CaMK1-beta antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

S. Huang

Verified customer

Asked: 2016-10-11

Answer

We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A11515 anti-CaMK1-beta antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2016-10-11

Order DetailsPrice
A11515

100 μL

$335

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A11515
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$335.00

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