Anti-Calponin 3/CNN3 Antibody Picoband™

Product Name

Anti-Calponin 3/CNN3 Antibody Picoband™

View all Acidic Calponin Antibodies

SKU/Catalog Number

A08267-4

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Calponin 3/CNN3 Antibody Picoband™ catalog # A08267-4. Tested in Flow Cytometry, WB applications. This antibody reacts with Human, Rat.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-Calponin 3/CNN3 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08267-4)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human Calponin 3/CNN3, identical to the related mouse and rat sequences.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A08267-4 is reactive to CNN3 in Human, Rat

Applications

A08267-4 is guaranteed for Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

39 kDa

Calculated molecular weight

Background of Acidic Calponin

Calponin 3. acidic is a protein that in humans is encoded by the CNN3 gene. This gene encodes a protein with a markedly acidic C terminus; the basic N-terminus is highly homologous to the N-terminus of a related gene, CNN1. Members of the CNN gene family all contain similar tandemly repeated motifs. This encoded protein is associated with the cytoskeleton but is not involved in contraction.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Rat

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of Calponin 3/CNN3 using anti-Calponin 3/CNN3 antibody (A08267-4).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: human U251 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Calponin 3/CNN3 antigen affinity purified polyclonal antibody (Catalog # A08267-4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Calponin 3/CNN3 at approximately 39 kDa. The expected band size for Calponin 3/CNN3 is at 39 kDa.

Click image to see more details

Figure 2. Flow Cytometry analysis of C6 cells using anti-Calponin 3/CNN3 antibody (A08267-4).
Overlay histogram showing C6 cells stained with A08267-4 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Calponin 3/CNN3 Antibody (A08267-4, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

Gene/Protein Information For CNN3 (Source: Uniprot.org, NCBI)

Alternative Names

calponin 3, acidic; Calponin, acidic isoform; calponin-3; dJ639P13.2.2 (acidic calponin 3) CNN3 calponin 3 calponin-3|calponin 3, acidic|dJ639P13.2.2 (acidic calponin 3)

For more info on CNN3, check out the CNN3 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for CNN3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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