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Product Info Summary
| SKU: | A03372-1 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, ICC, WB |
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Product info
Product Name
Anti-Calnexin CANX Antibody
SKU/Catalog Number
A03372-1
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Calnexin CANX Antibody catalog # A03372-1. Tested in ELISA, IF, IHC-P, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Calnexin CANX Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03372-1)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human Calnexin. AA range:543-592
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A03372-1 is reactive to CANX in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
67568 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03372-1 is guaranteed for ELISA, IF, IHC-P, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:5000
Validation Images & Assay Conditions
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Western Blot (WB) analysis of COLO205 cells using Calnexin Polyclonal antibody.
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Western Blot (WB) analysis of specific cells using Calnexin Polyclonal antibody.
Protein Target Info & Infographic
Gene/Protein Information For CANX (Source: Uniprot.org, NCBI)
Gene Name
CANX
Full Name
Calnexin
Weight
67568 MW
Superfamily
calreticulin family
Alternative Names
Calnexin;IP90;Major histocompatibility complex class I antigen-binding protein p88;p90;CANX; CANX CNX, IP90, P90 calnexin calnexin|epididymis secretory sperm binding protein|major histocompatibility complex class I -binding protein p88
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CANX, check out the CANX Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CANX: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Calnexin CANX Antibody (A03372-1)
Hello CJ!
A03372-1 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Yu F,Liang M,Huang Y,Wu W,Zheng B,Chen C.Hypoxic tumor-derived exosomal miR-31-5p promotes lung adenocarcinoma metastasis by negatively regulating SATB2-reversed EMT and activating MEK/ERK signaling.J Exp Clin Cancer Res.2021 Jun 1;40(1):179.doi:10.1186/s13046-021-01979-7.PMID:34074322.
Species: Human,Mouse
A03372-1 usage in article: APP:WB, SAMPLE:LUAD CELL, DILUTION:NA
Li Y,Yu Y,Xie Z,Ye X,Liu X,Xu B,Mao J.Serum-derived exosomes accelerate scald wound healing in mice by optimizing cellular functions and promoting Akt phosphorylation.Biotechnol Lett.2021 May 20.doi:10.1007/s10529-021-03148-4.Epub ahead of print.PMID:34014413.
Species: Mouse,Rat
A03372-1 usage in article: APP:WB, SAMPLE:SKIN TISSUE, DILUTION:1:1000
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Customer Reviews
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Customer Q&As
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5 Customer Q&As for Anti-Calnexin CANX Antibody
Question
I was wanting to use your anti-Calnexin antibody for WB for rat keratinocyte on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat keratinocyte identification?
Verified Customer
Verified customer
Asked: 2020-02-11
Answer
As indicated on the product datasheet, A03372-1 anti-Calnexin antibody has been tested for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat keratinocyte in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-02-11
Question
Will anti-Calnexin antibody A03372-1 work for WB with keratinocyte?
Verified Customer
Verified customer
Asked: 2019-04-09
Answer
According to the expression profile of keratinocyte, CANX is highly expressed in keratinocyte. So, it is likely that anti-Calnexin antibody A03372-1 will work for WB with keratinocyte.
Boster Scientific Support
Answered: 2019-04-09
Question
I would like to test anti-Calnexin antibody A03372-1 on rat keratinocyte for research purposes, then I may be interested in using anti-Calnexin antibody A03372-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-05-29
Answer
The products we sell, including anti-Calnexin antibody A03372-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-05-29
Question
Does A03372-1 anti-Calnexin antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2017-06-27
Answer
As indicated on the product datasheet, A03372-1 anti-Calnexin antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-06-27
Question
I see that the anti-Calnexin antibody A03372-1 works with WB, what is the protocol used to produce the result images on the product page?
C. Mangal
Verified customer
Asked: 2015-08-10
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2015-08-10
