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Product Info Summary
| SKU: | A05027 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-CA6/Carbonic Anhydrase Vi Antibody
View all Carbonic Anhydrase VI Antibodies
SKU/Catalog Number
A05027
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-CA6/Carbonic Anhydrase Vi Antibody (Catalog # A05027). Tested in WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CA6/Carbonic Anhydrase Vi Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05027)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human CA6.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A05027 is reactive to CA6 in Human
Reconstitution
Observed Molecular Weight
111 kDa
Calculated molecular weight
35367 MW
Background of Carbonic Anhydrase VI
Reversible hydration of carbon dioxide. Its role in saliva is unknown.
Aldred P., Biochemistry 30:569-575(1991).
Grubb D.J., Submitted (FEB-1999) to the EMBL/GenBank/DDBJ databases.
Gregory S.G., Nature 441:315-321(2006)
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05027 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Validation Images & Assay Conditions
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Western blot analysis of extracts from LOVO cells and HepG2 cells, using CA6 antibody A05027.
Protein Target Info & Infographic
Gene/Protein Information For CA6 (Source: Uniprot.org, NCBI)
Gene Name
CA6
Full Name
Carbonic anhydrase 6
Weight
35367 MW
Superfamily
alpha-carbonic anhydrase family
Alternative Names
Carbonic anhydrase 6;4.2.1.1;Carbonate dehydratase VI;Carbonic anhydrase VI;CA-VI;Salivary carbonic anhydrase;Secreted carbonic anhydrase;CA6; CA6 CA-VI, GUSTIN carbonic anhydrase 6 carbonic anhydrase 6|carbonate dehydratase VI|carbonic anhydrase VI nirs variant 2|salivary carbonic anhydrase|secreted carbonic anhydrase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CA6, check out the CA6 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CA6: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CA6/Carbonic Anhydrase Vi Antibody (A05027)
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No publications found for A05027
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Customer Reviews
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Customer Q&As
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12 Customer Q&As for Anti-CA6/Carbonic Anhydrase Vi Antibody
Question
Is this A05027 anti-CA6/Carbonic Anhydrase Vi antibody reactive to the isotypes of CA6?
Verified Customer
Verified customer
Asked: 2020-02-04
Answer
The immunogen of A05027 anti-CA6/Carbonic Anhydrase Vi antibody is Synthesized peptide derived from internal of human CA6. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-02-04
Question
Do you have a BSA free version of anti-CA6/Carbonic Anhydrase Vi antibody A05027 available?
Verified Customer
Verified customer
Asked: 2019-11-07
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-CA6/Carbonic Anhydrase Vi antibody A05027 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-11-07
Question
Our lab want to know about to test anti-CA6/Carbonic Anhydrase Vi antibody A05027 on human parotid gland for research purposes, then I may be interested in using anti-CA6/Carbonic Anhydrase Vi antibody A05027 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-10-09
Answer
The products we sell, including anti-CA6/Carbonic Anhydrase Vi antibody A05027, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-10-09
Question
Is a blocking peptide available for product anti-CA6/Carbonic Anhydrase Vi antibody (A05027)?
Verified Customer
Verified customer
Asked: 2019-10-09
Answer
We do provide the blocking peptide for product anti-CA6/Carbonic Anhydrase Vi antibody (A05027). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-10-09
Question
I was wanting to use your anti-CA6/Carbonic Anhydrase Vi antibody for WB for human parotid gland on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human parotid gland identification?
Verified Customer
Verified customer
Asked: 2019-07-24
Answer
As indicated on the product datasheet, A05027 anti-CA6/Carbonic Anhydrase Vi antibody has been validated for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human parotid gland in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-07-24
Question
Will anti-CA6/Carbonic Anhydrase Vi antibody A05027 work on dog WB with parotid gland?
Verified Customer
Verified customer
Asked: 2019-07-22
Answer
Our lab technicians have not tested anti-CA6/Carbonic Anhydrase Vi antibody A05027 on dog. You can run a BLAST between dog and the immunogen sequence of anti-CA6/Carbonic Anhydrase Vi antibody A05027 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated dog samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in dog parotid gland in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-07-22
Question
Please see the WB image, lot number and protocol we used for parotid gland using anti-CA6/Carbonic Anhydrase Vi antibody A05027. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-07-16
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-07-16
Question
Does anti-CA6/Carbonic Anhydrase Vi antibody A05027 work for WB with parotid gland?
Verified Customer
Verified customer
Asked: 2019-01-01
Answer
According to the expression profile of parotid gland, CA6 is highly expressed in parotid gland. So, it is likely that anti-CA6/Carbonic Anhydrase Vi antibody A05027 will work for WB with parotid gland.
Boster Scientific Support
Answered: 2019-01-01
Question
My question regarding product A05027, anti-CA6/Carbonic Anhydrase Vi antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-05-16
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A05027 anti-CA6/Carbonic Anhydrase Vi antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-05-16
Question
Would A05027 anti-CA6/Carbonic Anhydrase Vi antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2017-10-24
Answer
It shows on the product datasheet, A05027 anti-CA6/Carbonic Anhydrase Vi antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-10-24
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for parotid gland using anti-CA6/Carbonic Anhydrase Vi antibody A05027. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2017-09-06
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-09-06
Question
I see that the anti-CA6/Carbonic Anhydrase Vi antibody A05027 works with WB, what is the protocol used to produce the result images on the product page?
G. Anderson
Verified customer
Asked: 2015-05-19
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2015-05-19
