Product Info Summary
SKU: | A02057 |
---|---|
Size: | 100 µl |
Reactive Species: | Human |
Host: | Rabbit |
Application: | IHC, WB |
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Product info
Product Name
Anti-C1S/Complement Component C1S Antibody
View all Complement Component C1s Antibodies
SKU/Catalog Number
A02057
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-C1S/Complement Component C1S Antibody (Catalog # A02057). Tested in WB, IHC applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-C1S/Complement Component C1S Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02057)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human C1S.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A02057 is reactive to C1S in Human
Reconstitution
Calculated molecular weight
76684 MW
Background of Complement Component C1s
C1s B chain is a serine protease that combines with C1q and C1r to form C1, the first component of the classical pathway of the complement system. C1r activates C1s so that it can, in turn, activate C2 and C4.
McKinnon C.M., Eur. J. Biochem. 169:547-553(1987).
Tosi M., Biochemistry 26:8516-8524(1987).
Kusumoto H., Proc. Natl. Acad. Sci. U.S.A. 85:7307-7311(1988).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02057 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of extracts from Jurkat cells and COLO cells, using C1S antibody A02057.
Click image to see more details
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using C1S antibody A02057.
Protein Target Info & Infographic
Gene/Protein Information For C1S (Source: Uniprot.org, NCBI)
Gene Name
C1S
Full Name
Complement C1s subcomponent
Weight
76684 MW
Superfamily
peptidase S1 family
Alternative Names
Complement C1s subcomponent;3.4.21.42;C1 esterase;Complement component 1 subcomponent s;Complement C1s subcomponent heavy chain;Complement C1s subcomponent light chain;C1S; C1S EDSPD2 complement C1s complement C1s subcomponent|C1 esterase|basic proline-rich peptide IB-1|complement component 1 subcomponent s|complement component 1, s subcomponent
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on C1S, check out the C1S Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for C1S: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-C1S/Complement Component C1S Antibody (A02057)
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No publications found for A02057
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Customer Q&As
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6 Customer Q&As for Anti-C1S/Complement Component C1S Antibody
Question
We are currently using anti-C1S/Complement Component C1S antibody A02057 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on horse tissues as well?
L. Jha
Verified customer
Asked: 2020-04-17
Answer
The anti-C1S/Complement Component C1S antibody (A02057) has not been validated for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-04-17
Question
Is there a BSA free version of anti-C1S/Complement Component C1S antibody A02057 available?
Verified Customer
Verified customer
Asked: 2020-01-09
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-C1S/Complement Component C1S antibody A02057 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-01-09
Question
Is a blocking peptide available for product anti-C1S/Complement Component C1S antibody (A02057)?
Verified Customer
Verified customer
Asked: 2019-07-11
Answer
We do provide the blocking peptide for product anti-C1S/Complement Component C1S antibody (A02057). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-07-11
Question
My lab would like to test anti-C1S/Complement Component C1S antibody A02057 on human pns for research purposes, then I may be interested in using anti-C1S/Complement Component C1S antibody A02057 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
P. Carter
Verified customer
Asked: 2018-12-11
Answer
The products we sell, including anti-C1S/Complement Component C1S antibody A02057, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-12-11
Question
See attached the WB image, lot number and protocol we used for pns using anti-C1S/Complement Component C1S antibody A02057. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2017-08-24
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-08-24
Question
Will anti-C1S/Complement Component C1S antibody A02057 work on dog IHC with peripheral blood leukocyte?
G. Li
Verified customer
Asked: 2013-05-15
Answer
Our lab technicians have not tested anti-C1S/Complement Component C1S antibody A02057 on dog. You can run a BLAST between dog and the immunogen sequence of anti-C1S/Complement Component C1S antibody A02057 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated dog samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in dog peripheral blood leukocyte in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2013-05-15