Anti-C16orf75/RMI2 Antibody Picoband™

Product Name

Anti-C16orf75/RMI2 Antibody Picoband™

View all RMI2 Antibodies

SKU/Catalog Number

A08685-1

Size

100 µg/vial

Form

Lyophilized

Description

Boster Bio Anti-C16orf75/RMI2 Antibody Picoband™ catalog # A08685-1. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-C16orf75/RMI2 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08685-1)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

IgG

Immunogen

E.coli-derived human C16orf75/RMI2 recombinant protein (Position: L23-P147). Human RMI2 shares 87.2% amino acid (aa) sequence identity with mouse RMI2.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A08685-1 is reactive to RMI2 in Human, Mouse, Rat

Applications

A08685-1 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

16 kDa

Calculated molecular weight

52588 MW

Background of RMI2

RMI2 is a component of the BLM (RECQL3) complex, which plays a role in homologous recombination-dependent DNA repair and is essential for genome stability. This gene is mapped to 16p13.13. RMI1 and RMI2 were present in approximately stoichiometric amounts with other BLM complex components, including topoisomerase-3-alpha (TOP3A), RPA (see RPA1), and BLAP250. RMI2 also associated with RMI1 and TOP3A in a second complex. RMI1 and RMI2 interacted directly, and both were essential for stability of the BLM complex. Depletion of either RMI1 or RMI2 depleted the other protein by 80 to 90%. Chicken DT40 cells depleted of Rmi2 displayed elevated sister chromatid exchange, but other functions of the BLM complex appeared intact. Mutation analysis revealed that interaction between human RMI2 and BLM was essential for suppression of sister chromatid exchange.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Human, Mouse, Rat
ELISA, 0.1-0.5 µg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of C16orf75/RMI2 using anti-C16orf75/RMI2 antibody (A08685-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: human MCF-7 whole cell lysates,
Lane 3: human HT-1080 whole cell lysates,
Lane 4: human Hela whole cell lysates,
Lane 5: rat thymus tissue lysates,
Lane 6: mouse thymus tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-C16orf75/RMI2 antigen affinity purified polyclonal antibody (Catalog # A08685-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for C16orf75/RMI2 at approximately 16 kDa. The expected band size for C16orf75/RMI2 is at 16 kDa.

Gene/Protein Information For RMI2 (Source: Uniprot.org, NCBI)

Alternative Names

BLAP18RMI2; BLM-associated protein of 18 kDa; chromosome 16 open reading frame 75; hRMI2; MGC24665; RecQ-mediated genome instability 2, S. cerevisiae, homolog of; recQ-mediated genome instability protein 2 RMI2 BLAP18, C16orf75 RecQ mediated genome instability 2 recQ-mediated genome instability protein 2|BLM-associated protein of 18 kDa|RMI2, RecQ mediated genome instability 2, homolog

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