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Product Info Summary
| SKU: | MA1028 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | IHC, ICC, WB |
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Product info
Product Name
Anti-c-Myc Antibody (Monoclonal, 9E10)
SKU/Catalog Number
MA1028
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-c-Myc Antibody (Monoclonal, 9E10) catalog # MA1028. Tested in IHC, ICC, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-c-Myc Antibody (Monoclonal, 9E10) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1028)
Host
Mouse
Contents
Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
Clonality
Monoclonal
Clone Number
Clone: 9E10
Isotype
Mouse IgG1
Immunogen
Synthetic peptide corresponding to residues 408-439 of the human p62c-Myc protein.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
MA1028 is reactive to Myc in Human
Reconstitution
Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Observed Molecular Weight
49 kDa
Calculated molecular weight
48898 MW
Background of c-Myc
C-Myc is an oncogene that functions both in the stimulation of cell proliferation and in apoptosis. c-Myc elicits its oncogenic activity by causing immortalization, and to a lesser extent the transformation of cells, in addition to several other mechanisms. The c-MYC proto-oncogene encodes a transcription factor that is critical for cell growth and proliferation. It is one of the genes frequently altered in cancer cells in which it exhibits constitutive activity. Downregulation of c-Myc is critical for 2-Methoxyestradiol (2ME2)-induced oxidative stress and apoptosis in AML cells. And its up-regulation is important for promoting lymphocyte cell division, and demonstrating that GFP-c-Myc expression is a marker of proliferating lymphocytes in vivo.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
MA1028 is guaranteed for IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 5μg/ml, Human, By Heat
Immunocytochemistry , 1μg/ml, Human, -
Western blot, 1μg/ml, Human
Positive Control
WB: human Hela whole cell
IHC: human spleen tissue, human colorectal adenocarcinoma tissue, human thyroid cancer tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of c-Myc using anti-c-Myc antibody (MA1028).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-c-Myc antigen affinity purified monoclonal antibody (Catalog # MA1028) at 1 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for c-Myc at approximately 49 kDa. The expected band size for c-Myc is at 49 kDa.
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Figure 2. IHC analysis of c-Myc using anti-c-Myc antibody (MA1028).
c-Myc was detected in paraffin-embedded section of human spleen tissues. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5μg/ml mouse anti-c-Myc Antibody (MA1028) overnight at 4°C. Peroxidase Conjugated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of c-Myc using anti-c-Myc antibody (MA1028).
c-Myc was detected in paraffin-embedded section of human colorectal adenocarcinoma tissues. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5μg/ml mouse anti-c-Myc Antibody (MA1028) overnight at 4°C. Peroxidase Conjugated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.
Click image to see more details
Figure 4. IHC analysis of c-Myc using anti-c-Myc antibody (MA1028).
c-Myc was detected in paraffin-embedded section of human thyroid cancer tissues. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5μg/ml mouse anti-c-Myc Antibody (MA1028) overnight at 4°C. Peroxidase Conjugated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For Myc (Source: Uniprot.org, NCBI)
Gene Name
Myc
Full Name
Myc proto-oncogene protein
Weight
48898 MW
Alternative Names
Myc proto-oncogene protein;Proto-oncogene c-Myc;Transcription factor p64;Myc; MYC MRTLC, bHLHe39, c-Myc, MYC MYC proto-oncogene, bHLH transcription factor myc proto-oncogene protein|avian myelocytomatosis viral oncogene homolog|class E basic helix-loop-helix protein 39|myc-related translation/localization regulatory factor|proto-oncogene c-Myc|transcription factor p64|v-myc avian myelocytomatosis viral oncogene homolog|v-myc myelocytomatosis viral oncogene homolog
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Myc, check out the Myc Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Myc: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-c-Myc Antibody (Monoclonal, 9E10) (MA1028)
Hello CJ!
MA1028 has been cited in 16 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Long non-coding RNA LINC01503 predicts worse prognosis in glioma and promotes tumorigenesis and progression through activation of Wnt/β-catenin signaling H Wang 1, Z-G Sheng, L-Z Dai Eur Rev Med Pharmacol Sci. 2019 Feb;23(4):1600-1609. doi: 10.26355/e
Inhibition of N-myc downstream-regulated gene 2 in prostatic carcinoma
Arginine deiminase expressed in vivo, driven by human telomerase reverse transcriptase promoter, displays high hepatoma targeting and oncolytic efficiency
Tumor suppressor NDRG2 inhibits glycolysis and glutaminolysis in colorectal cancer cells by repressing c-Myc expression
Blockage of Glyoxalase I Inhibits Colorectal Tumorigenesis and Tumor Growth via Upregulation of STAT1, p53, and Bax and Downregulation of c-Myc and Bcl-2
Wang Ph, Gu Zh, Wan Dh, Zhu Wb, Qiu W, Weng Sp, Yu Xq, He Jg. Plos One. 2013;8(2):E52088. Doi: 10.1371/Journal.Pone.0052088. Epub 2013 Feb 6. Litopenaeus Vannamei Sterile-Alpha And Armadillo Motif Containing Protein (Lvsarm) Is Involved In Regulat...
Long Xb, Sun Gb, Hu S, Liang Gt, Wang N, Zhang Xh, Cao Pp, Zhen Ht, Cui Yh, Liu Z. Oncol Rep. 2009 Nov;22(5):1189-95. Let-7A Microrna Functions As A Potential Tumor Suppressor In Human Laryngeal Cancer.
Liu B, Liu F, Bai L, Li Y, Yang G. Mol Biol Rep. 2010 Mar;37(3):1671-6. Doi: 10.1007/S11033-009-9858-Y. Epub 2009 Oct 13. Molecular Cloning, Expression And Subcellular Distribution Of An Alternative Splice Variant Of The Porcine Sirt2 Gene.
Zhang K, Lu Y, Yang P, Li C, Sun H, Tao D, Liu Y, Zhang S, Ma Y. Plos One. 2012;7(7):E41973. Doi: 10.1371/Journal.Pone.0041973. Epub 2012 Jul 27. Hili Inhibits Tgf-?? Signaling By Interacting With Hsp90 And Promoting T??r Degradation.
Hao Hp, Wen Lb, Li Jr, Wang Y, Ni B, Wang R, Wang X, Sun Mx, Fan Hj, Mao X. Antiviral Res. 2015 May;117:99-109. Doi: 10.1016/J.Antiviral.2015.02.010. Epub 2015 Mar 5. Licl Inhibits Prrsv Infection By Enhancing Wnt/??-Catenin Pathway And Suppressin...
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Customer Reviews
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Customer Q&As
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17 Customer Q&As for Anti-c-Myc Antibody (Monoclonal, 9E10)
Question
I have attached the WB image, lot number and protocol we used for promyelocytic leukemia using anti-c-Myc antibody (Monoclonal, 9E10) MA1028. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-04-23
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-04-23
Question
I see that the anti-c-Myc antibody (Monoclonal, 9E10) MA1028 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-02-07
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-02-07
Question
Will MA1028 anti-c-Myc antibody (Monoclonal, 9E10) work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-11-19
Answer
As indicated on the product datasheet, MA1028 anti-c-Myc antibody (Monoclonal, 9E10) as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-11-19
Question
you antibody to test anti-c-Myc antibody (Monoclonal, 9E10) MA1028 on human promyelocytic leukemia for research purposes, then I may be interested in using anti-c-Myc antibody (Monoclonal, 9E10) MA1028 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
R. Li
Verified customer
Asked: 2019-09-27
Answer
The products we sell, including anti-c-Myc antibody (Monoclonal, 9E10) MA1028, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-09-27
Question
We bought anti-c-Myc antibody (Monoclonal, 9E10) for WB on cervix a few years ago. I am using human, and We intend to use the antibody for IHC next. I am interested in examining cervix as well as cervix carcinoma erythroleukemia in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC?
Verified Customer
Verified customer
Asked: 2019-09-24
Answer
I have checked the website and datasheets of our anti-c-Myc antibody (Monoclonal, 9E10) and it seems that MA1028 has been tested on human in both WB and IHC. Thus MA1028 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-09-24
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for promyelocytic leukemia using anti-c-Myc antibody (Monoclonal, 9E10) MA1028. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-06-26
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-06-26
Question
Will anti-c-Myc antibody (Monoclonal, 9E10) MA1028 work for IHC with promyelocytic leukemia?
Verified Customer
Verified customer
Asked: 2019-05-24
Answer
According to the expression profile of promyelocytic leukemia, MYC is highly expressed in promyelocytic leukemia. So, it is likely that anti-c-Myc antibody (Monoclonal, 9E10) MA1028 will work for IHC with promyelocytic leukemia.
Boster Scientific Support
Answered: 2019-05-24
Question
Is a blocking peptide available for product anti-c-Myc antibody (Monoclonal, 9E10) (MA1028)?
Verified Customer
Verified customer
Asked: 2018-04-09
Answer
We do provide the blocking peptide for product anti-c-Myc antibody (Monoclonal, 9E10) (MA1028). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-04-09
Question
Is this MA1028 anti-c-Myc antibody (Monoclonal, 9E10) reactive to the isotypes of MYC?
Verified Customer
Verified customer
Asked: 2018-02-09
Answer
The immunogen of MA1028 anti-c-Myc antibody (Monoclonal, 9E10) is Synthetic peptide corresponding to residues 408-439 of the human p62c-Myc protein. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-02-09
Question
Is there a BSA free version of anti-c-Myc antibody (Monoclonal, 9E10) MA1028 available?
A. Zhao
Verified customer
Asked: 2017-12-21
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-c-Myc antibody (Monoclonal, 9E10) MA1028 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-12-21
Question
My question regarding product MA1028, anti-c-Myc antibody (Monoclonal, 9E10). I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2017-12-15
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free MA1028 anti-c-Myc antibody (Monoclonal, 9E10), we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-12-15
Question
Will anti-c-Myc antibody (Monoclonal, 9E10) MA1028 work on zebrafish IHC with cervix?
C. Wu
Verified customer
Asked: 2017-03-29
Answer
Our lab technicians have not validated anti-c-Myc antibody (Monoclonal, 9E10) MA1028 on zebrafish. You can run a BLAST between zebrafish and the immunogen sequence of anti-c-Myc antibody (Monoclonal, 9E10) MA1028 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated zebrafish samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in zebrafish cervix in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-03-29
Question
We need using your anti-c-Myc antibody (Monoclonal, 9E10) for response to gamma radiation studies. Has this antibody been tested with western blotting on cardiac muscle tissue? We would like to see some validation images before ordering.
N. Dhar
Verified customer
Asked: 2016-12-30
Answer
Thank you for your inquiry. This MA1028 anti-c-Myc antibody (Monoclonal, 9E10) is validated on cardiac muscle tissue. It is guaranteed to work for IHC, ICC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2016-12-30
Question
I was wanting to use your anti-c-Myc antibody (Monoclonal, 9E10) for IHC for human promyelocytic leukemia on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human promyelocytic leukemia identification?
J. Roberts
Verified customer
Asked: 2015-05-14
Answer
As indicated on the product datasheet, MA1028 anti-c-Myc antibody (Monoclonal, 9E10) has been tested for IHC, ICC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human promyelocytic leukemia in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2015-05-14
Question
We are currently using anti-c-Myc antibody (Monoclonal, 9E10) MA1028 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on zebrafish tissues as well?
K. Banerjee
Verified customer
Asked: 2014-11-24
Answer
The anti-c-Myc antibody (Monoclonal, 9E10) (MA1028) has not been validated for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2014-11-24
Question
We have observed staining in human cervix. What should we do? Is anti-c-Myc antibody (Monoclonal, 9E10) supposed to stain cervix positively?
G. Yang
Verified customer
Asked: 2014-09-05
Answer
From what I have seen in literature cervix does express MYC. From what I have seen in Uniprot.org, MYC is expressed in thoracic mammary gland, uterus, cervix, placenta testis, promyelocytic leukemia, cervix carcinoma, cervix carcinoma erythroleukemia, among other tissues. Regarding which tissues have MYC expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Cervix, Placenta, and Testis, Pubmed ID: 15489334
Promyelocytic leukemia, Pubmed ID: 3540591
Uterus, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2014-09-05
Question
We were satisfied with the WB result of your anti-c-Myc antibody (Monoclonal, 9E10). However we have been able to see positive staining in cervix carcinoma erythroleukemia nucleoplasm using this antibody. Is that expected? Could you tell me where is MYC supposed to be expressed?
C. Thomas
Verified customer
Asked: 2013-01-22
Answer
According to literature, cervix carcinoma erythroleukemia does express MYC. Generally MYC expresses in nucleus, nucleoplasm. Regarding which tissues have MYC expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Cervix, Placenta, and Testis, Pubmed ID: 15489334
Promyelocytic leukemia, Pubmed ID: 3540591
Uterus, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2013-01-22
