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Product Info Summary
| SKU: | M00026-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-c-Myc Monoclonal Antibody
SKU/Catalog Number
M00026-1
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-c-Myc Monoclonal Antibody catalog # M00026-1. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-c-Myc Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00026-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AHI-13
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human c-Myc Transcription factor that binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Activates the transcription of growth-related genes. Myc a proto-oncogenic transcription factor that plays a role in cell proliferation, apoptosis and in the development of human tumors.. Seems to activate the transcription of growth-related genes.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00026-1 is reactive to MYC in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
56 kDa
Calculated molecular weight
88068 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00026-1 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
FC 1:50
Positive Control
IHC: human lung cancer tissue
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:1W dilution for 1 hour at room temperature.
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Immunohistochemical analysis of paraffin-embedded human lung cancer, using c-Myc Antibody.
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
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Immunofluorescent analysis of HeLa cells, using c-Myc Antibody.
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Western blot analysis of c-Myc expression in (1) Jurkat cell lysate;(2) K562 cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For MYC (Source: Uniprot.org, NCBI)
Gene Name
MYC
Full Name
Myc proto-oncogene protein
Weight
88068 MW
Alternative Names
Signal transducer and activator of transcription 3;Acute-phase response factor;STAT3;APRF; MYC MRTLC, bHLHe39, c-Myc, MYC MYC proto-oncogene, bHLH transcription factor myc proto-oncogene protein|avian myelocytomatosis viral oncogene homolog|class E basic helix-loop-helix protein 39|myc-related translation/localization regulatory factor|proto-oncogene c-Myc|transcription factor p64|v-myc avian myelocytomatosis viral oncogene homolog|v-myc myelocytomatosis viral oncogene homolog
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MYC, check out the MYC Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MYC: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-c-Myc Monoclonal Antibody (M00026-1)
Hello CJ!
M00026-1 has been cited in 8 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Constitutive %u03B2-catenin activation in osteoblasts impairs terminal osteoblast differentiation and bone quality
Inhibition of N-myc downstream-regulated gene 2 in prostatic carcinoma
Tumor suppressor NDRG2 inhibits glycolysis and glutaminolysis in colorectal cancer cells by repressing c-Myc expression
Metformin Inhibits Tumorigenesis and Tumor Growth of Breast Cancer Cells by Upregulating miR-200c but Downregulating AKT2 Expression
Blockage of Glyoxalase I Inhibits Colorectal Tumorigenesis and Tumor Growth via Upregulation of STAT1, p53, and Bax and Downregulation of c-Myc and Bcl-2
Zhang D, Fei F, Li S, Zhao Y, Yang Z, Qu J, Zhang X, Yin Y, Zhang S. J Cancer. 2017 Jul 5;8(11):2114-2123. doi: 10.7150/jca.19723. eCollection 2017. The role of β-catenin in the initiation and metastasis of TA2 mice spontaneous breast cancer
Li M, Tian L, Wang L, Yao H, Zhang J, Lu J, Sun Y, Gao X, Xiao H, Liu M. Plos One. 2013 Oct 23;8(10):E77829. Doi: 10.1371/Journal.Pone.0077829. Ecollection 2013. Down-Regulation Of Mir-129-5P Inhibits Growth And Induces Apoptosis In Laryngeal Squa...
Yang H, Gu J, Zheng Q, Li M, Lian X, Miao J, Jiang J, Wei W. J Biol Chem. 2011 Apr 1;286(13):11865-74. Doi: 10.1074/Jbc.M110.136929. Epub 2011 Feb 10. Rpb5-Mediating Protein Is Required For The Proliferation Of Hepatocellular Carcinoma Cells.
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Customer Reviews
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Customer Q&As
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14 Customer Q&As for Anti-c-Myc Monoclonal Antibody
Question
Is a blocking peptide available for product anti-c-Myc Monoclonal antibody (M00026-1)?
Verified Customer
Verified customer
Asked: 2020-03-23
Answer
We do provide the blocking peptide for product anti-c-Myc Monoclonal antibody (M00026-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-03-23
Question
Do you have a BSA free version of anti-c-Myc Monoclonal antibody M00026-1 available?
Verified Customer
Verified customer
Asked: 2019-11-01
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-c-Myc Monoclonal antibody M00026-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-11-01
Question
I see that the anti-c-Myc Monoclonal antibody M00026-1 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-10-23
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-10-23
Question
My question regarding product M00026-1, anti-c-Myc Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-09-02
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00026-1 anti-c-Myc Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-09-02
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix using anti-c-Myc Monoclonal antibody M00026-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-08-09
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-08-09
Question
Will M00026-1 anti-c-Myc Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-08-05
Answer
It shows on the product datasheet, M00026-1 anti-c-Myc Monoclonal antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-08-05
Question
I was wanting to use your anti-c-Myc Monoclonal antibody for WB for mouse cervix on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse cervix identification?
Verified Customer
Verified customer
Asked: 2019-05-23
Answer
You can see on the product datasheet, M00026-1 anti-c-Myc Monoclonal antibody has been validated for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse cervix in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-05-23
Question
Will anti-c-Myc Monoclonal antibody M00026-1 work for WB with cervix?
O. Gonzalez
Verified customer
Asked: 2019-04-29
Answer
According to the expression profile of cervix, MYC is highly expressed in cervix. So, it is likely that anti-c-Myc Monoclonal antibody M00026-1 will work for WB with cervix.
Boster Scientific Support
Answered: 2019-04-29
Question
We are currently using anti-c-Myc Monoclonal antibody M00026-1 for mouse tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2019-04-11
Answer
The anti-c-Myc Monoclonal antibody (M00026-1) has not been tested for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-04-11
Question
Our lab were well pleased with the WB result of your anti-c-Myc Monoclonal antibody. However we have observed positive staining in cervix carcinoma erythroleukemia nucleus using this antibody. Is that expected? Could you tell me where is MYC supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-02-05
Answer
Based on literature, cervix carcinoma erythroleukemia does express MYC. Generally MYC expresses in nucleus, nucleoplasm. Regarding which tissues have MYC expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Cervix, Placenta, and Testis, Pubmed ID: 15489334
Promyelocytic leukemia, Pubmed ID: 3540591
Uterus, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2019-02-05
Question
I have attached the WB image, lot number and protocol we used for cervix using anti-c-Myc Monoclonal antibody M00026-1. Please let me know if you require anything else.
K. Baker
Verified customer
Asked: 2018-05-08
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-05-08
Question
We have been able to see staining in human cervix carcinoma erythroleukemia. Are there any suggestions? Is anti-c-Myc Monoclonal antibody supposed to stain cervix carcinoma erythroleukemia positively?
Verified Customer
Verified customer
Asked: 2017-12-26
Answer
According to literature cervix carcinoma erythroleukemia does express MYC. According to Uniprot.org, MYC is expressed in thoracic mammary gland, uterus, cervix, placenta testis, promyelocytic leukemia, cervix carcinoma, cervix carcinoma erythroleukemia, among other tissues. Regarding which tissues have MYC expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Cervix, Placenta, and Testis, Pubmed ID: 15489334
Promyelocytic leukemia, Pubmed ID: 3540591
Uterus, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2017-12-26
Question
you antibody using your anti-c-Myc Monoclonal antibody for cellular iron ion homeostasis studies. Has this antibody been tested with western blotting on jurkat cell lysate? We would like to see some validation images before ordering.
B. Jackson
Verified customer
Asked: 2014-12-26
Answer
I appreciate your inquiry. This M00026-1 anti-c-Myc Monoclonal antibody is tested on hela cells, jurkat cell lysate, k562 cell lysate. It is guaranteed to work for WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2014-12-26
Question
I am interested in to test anti-c-Myc Monoclonal antibody M00026-1 on mouse cervix for research purposes, then I may be interested in using anti-c-Myc Monoclonal antibody M00026-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
J. Zhao
Verified customer
Asked: 2013-12-26
Answer
The products we sell, including anti-c-Myc Monoclonal antibody M00026-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2013-12-26
