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Product Info Summary
| SKU: | M00297 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, WB |
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Product info
Product Name
Anti-c-Fos Rabbit Monoclonal Antibody
SKU/Catalog Number
M00297
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-c-Fos Rabbit Monoclonal Antibody catalog # M00297. Tested in WB, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-c-Fos Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00297)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
IIO-6
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human c-Fos
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00297 is reactive to FOS in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
3 kDa
Calculated molecular weight
40695 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00297 is guaranteed for Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
FC 1:50
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:5K dilution for 1 hour at room temperature.
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Western blot analysis of c-Fos expression in HeLa cell lysate treated with TPA.
Protein Target Info & Infographic
Gene/Protein Information For FOS (Source: Uniprot.org, NCBI)
Gene Name
FOS
Full Name
Proto-oncogene c-Fos
Weight
40695 MW
Superfamily
bZIP family
Alternative Names
Proto-oncogene c-Fos;Cellular oncogene fos;G0/G1 switch regulatory protein 7;FOS;G0S7; FOS AP-1, C-FOS, p55 Fos proto-oncogene, AP-1 transcription factor subunit proto-oncogene c-Fos|FBJ murine osteosarcoma viral (v-fos) oncogene homolog (oncogene FOS)|FBJ murine osteosarcoma viral oncogene homolog|Fos proto-oncogene, AP-1 trancription factor subunit|G0/G1 switch regulatory protein 7|activator protein 1|cellular oncogene c-fos
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on FOS, check out the FOS Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for FOS: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-c-Fos Rabbit Monoclonal Antibody (M00297)
Hello CJ!
M00297 has been cited in 6 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Overexpression of c-fos in Helicobacter pylori-induced gastric precancerosis of Mongolian gerbil
Changes in the expression of c-fos & heat shock protein genes & blood flow velocity in the brain of rats undergoing myocardial ischaemia/reperfusion
Positional and expressive alteration of prohibitin during the induced differentiation of human hepatocarcinoma SMMC-7721 cells
Analgesic Neural Circuits Are Activated by Electroacupuncture at Two Sets of Acupoints
miR-144 and targets, c-fos and cyclooxygenase-2 (COX2), modulate synthesis of PGE2 in the amnion during pregnancy and labor
The Expression Patterns of c-Fos and c-Jun Induced by Different Frequencies of Electroacupuncture in the Brain
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Anti-c-Fos Rabbit Monoclonal Antibody
Question
We have seen staining in rat pancreas. What should we do? Is anti-c-Fos Rabbit Monoclonal antibody supposed to stain pancreas positively?
Verified Customer
Verified customer
Asked: 2020-03-16
Answer
According to literature pancreas does express FOS. According to Uniprot.org, FOS is expressed in mucosa of stomach, colon, pancreas, leukemic t-cell, among other tissues. Regarding which tissues have FOS expression, here are a few articles citing expression in various tissues:
Colon, Pubmed ID: 14702039
Leukemic T-cell, Pubmed ID: 19690332
Pancreas, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2020-03-16
Question
We want using your anti-c-Fos Rabbit Monoclonal antibody for response to gravity studies. Has this antibody been tested with western blotting on hela cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2018-02-21
Answer
Thank you for your inquiry. This M00297 anti-c-Fos Rabbit Monoclonal antibody is tested on hela cell lysate. It is guaranteed to work for WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2018-02-21
Question
My colleagues were content with the WB result of your anti-c-Fos Rabbit Monoclonal antibody. However we have observed positive staining in mucosa of stomach nucleus. endoplasmic reticulum. cytoplasm, using this antibody. Is that expected? Could you tell me where is FOS supposed to be expressed?
S. Anderson
Verified customer
Asked: 2016-03-14
Answer
From literature, mucosa of stomach does express FOS. Generally FOS expresses in nucleus. endoplasmic reticulum. cytoplasm,. Regarding which tissues have FOS expression, here are a few articles citing expression in various tissues:
Colon, Pubmed ID: 14702039
Leukemic T-cell, Pubmed ID: 19690332
Pancreas, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2016-03-14
Question
We are currently using anti-c-Fos Rabbit Monoclonal antibody M00297 for rat tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on pig tissues as well?
K. Taylor
Verified customer
Asked: 2015-12-28
Answer
The anti-c-Fos Rabbit Monoclonal antibody (M00297) has not been validated for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2015-12-28
