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Product Info Summary
| SKU: | M13975 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | Flow Cytometry, IF, IHC, WB |
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Product info
Product Name
Anti-Breast cancer suppressor candidate 1 (VWA5A) Mouse Monoclonal Antibody [Clone ID: OTI3D6]
View all Breast cancer suppressor candidate 1 Antibodies
SKU/Catalog Number
M13975
Size
100 µl
Description
Boster Bio VWA5A mouse monoclonal antibody, clone OTI3D6 (formerly 3D6). Catalog# M13975. Tested in FC, IF, IHC, WB. This antibody reacts with Human.
Storage & Handling
Store at -20°C as received.
Cite This Product
Anti-Breast cancer suppressor candidate 1 (VWA5A) Mouse Monoclonal Antibody [Clone ID: OTI3D6] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M13975)
Host
Mouse
Contents
PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Clonality
Monoclonal
Clone Number
OTI3D6
Isotype
IgG2a
Immunogen
Full length human recombinant protein of human VWA5A (NP_055437) produced in HEK293T cell.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M13975 is reactive to VWA5A in Human
Applications
M13975 is guaranteed for Flow Cytometry, IF, IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:2000
IHC 1:150
IF 1:100
FCM 1:100
Validation Images & Assay Conditions
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VWA5A (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VWA5A.
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IHC analysis of VWA5A using anti-VWA5A antibody (M13975).
VWA5A was detected in a paraffin-embedded section of human colorectal adenocarcinom tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:200 mouse anti-VWA5A Antibody (M13975) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.
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IHC analysis of VWA5A using anti-VWA5A antibody (M13975).
VWA5A was detected in a paraffin-embedded section of human esophageal squamous carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:200 mouse anti-VWA5A Antibody (M13975) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.
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Anti-VWA5A mouse monoclonal antibody (M13975) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY VWA5A.
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Flow cytometric Analysis of Hela cells
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Flow cytometric Analysis of Jurkat cells
Protein Target Info & Infographic
Gene/Protein Information For VWA5A (Source: Uniprot.org, NCBI)
Gene Name
VWA5A
Full Name
von Willebrand factor A domain-containing protein 5A
Weight
Alternative Names
BCSC-1Loss of heterozygosity 11 chromosomal region 2 gene A protein; BCSC1ortholog of mouse AW551984; Breast cancer suppressor candidate 1; LOH11CR2Avon Willebrand factor A domain-containing protein 5A; loss of heterozygosity, 11, chromosomal region 2, gene A; von Willebrand factor A domain containing 5A VWA5A BCSC-1, BCSC1, LOH11CR2A von Willebrand factor A domain containing 5A von Willebrand factor A domain-containing protein 5A|breast cancer suppressor candidate 1|loss of heterozygosity 11 chromosomal region 2 gene A protein|loss of heterozygosity, 11, chromosomal region 2, gene A|ortholog of mouse AW551984
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on VWA5A, check out the VWA5A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for VWA5A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Breast cancer suppressor candidate 1 (VWA5A) Mouse Monoclonal Antibody [Clone ID: OTI3D6] (M13975)
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