Anti-BRAF35 HMG20B Antibody

Product Name

Anti-BRAF35 HMG20B Antibody

View all BRAF35 Antibodies

SKU/Catalog Number

A08646

Size

100μl

Form

Liquid

Description

Boster Bio Anti-BRAF35 HMG20B Antibody catalog # A08646. Tested in WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-BRAF35 HMG20B Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08646)

Host

Rabbit

Contents

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from the N-terminal region of human BRAF35. at AA rangle: 10-90

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A08646 is reactive to HMG20B in Human

Applications

A08646 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

39 kDa

Calculated molecular weight

35813 MW

Background of BRAF35

APC11 is also known as Anaphase promoting complex subunit 11, APC11, Cyclosome subunit 11, Hepatocellular carcinoma associated RING finger protein, and HSPC214. APC11 is a component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G₁ phase of the cell cycle. APC11 may function to recruit the E2 ubiquitin-conjugating enzymes to the complex. APC11 interacts with the cullin domain of ANAPC2 and also interacts with UBE2D2. APC11 shows both a cytoplasmic and nuclear localization. APC11 is expressed at high levels in skeletal muscle and heart; in moderate levels in brain, kidney, and liver; and at low levels in colon, thymus, spleen, small intestine, placenta, lung and peripheral blood leukocyte. APC11 is a member of the RING-type zinc finger family and is auto-ubiquitinylated.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB, 1:500-1:2000

Validation Images & Assay Conditions

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Western Blot (WB) analysis of AD293 cells using BRAF35 Polyclonal antibody.

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Western Blot (WB) analysis of specific cells using BRAF35 Polyclonal antibody.

Gene/Protein Information For HMG20B (Source: Uniprot.org, NCBI)

Alternative Names

BRAF35Sox-like transcriptional factor; high-mobility group 20B; HMG domain-containing protein 2; HMG domain-containing protein HMGX2; HMGX2FLJ26127; HMGXB2member 1-related; SMARCE1R; SMARCE1-related protein HMG20B BRAF25, BRAF35, HMGX2, HMGXB2, PP7706, SMARCE1r, SOXL, pp8857 high mobility group 20B SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily E member 1-related|BRCA2-associated factor 35|HMG box domain containing 2|HMG box-containing protein 20B|HMG domain-containing protein 2|HMG domain-containing protein HMGX2|SMARCE1-related protein|SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily E, member 1-related|Sox-like transcriptional factor|structural DNA-binding protein BRAF35

For more info on HMG20B, check out the HMG20B Infographic

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In this infographic, you will see the following information for HMG20B: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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