Click image to see more details
Product Info Summary
| SKU: | A05724 |
|---|---|
| Size: | 100µg |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Bradykinin Receptor B1 Bdkrb1 Antibody
View all Bradykinin RB1/BDKRB1 Antibodies
SKU/Catalog Number
A05724
Size
100µg
Form
Liquid. In PBS containing 50% glycerol and 0.01% sodium azide.
Description
Boster Bio Anti-Bradykinin Receptor B1 Bdkrb1 Antibody catalog # A05724. Tested in ELISA, WB applications. This antibody reacts with Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Bradykinin Receptor B1 Bdkrb1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05724)
Host
Rabbit
Contents
Liquid. In PBS containing 50% glycerol and 0.01% sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
Synthetic peptide corresponding to the sequence near the N-terminus of rat bradykinin receptor B1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A05724 is reactive to Bdkrb1 in Mouse, Rat
Reconstitution
Calculated molecular weight
43204 MW
Background of Bradykinin RB1/BDKRB1
SmarcAL1 (SWI/SNF-related µatrix-associated actin-dependent regulator of chromatin a-like 1) is also known as HARP, HepA Related Protein, HHARP, SMARCA like Protein 1 and SWI/SNF Related. SmarcAL1 is a member of the SWI/SNF family of proteins. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein shows sequence similarity to the E. coli RNA polymerase-binding protein HepA. Mutations in this gene are a cause of Schimke immunoosseous dysplasia (SIOD), an autosomal recessive disorder with the diagnostic features of spondyloepiphyseal dysplasia, renal dysfunction, and T-cell immunodeficiency.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05724 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Optimal conditions must be determined individually for each application.
Validation Images & Assay Conditions
Click image to see more details
Membrane ELISA: Lewis rat heart membranes (5µ
Click image to see more details
Figure 1. Western blot analysis of Bdkrb1 using anti-Bdkrb1 antibody (A05724).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Bdkrb1 antigen affinity purified polyclonal antibody (Catalog # A05724) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for Bdkrb1.
Protein Target Info & Infographic
Gene/Protein Information For Bdkrb1 (Source: Uniprot.org, NCBI)
Gene Name
Bdkrb1
Full Name
B1 bradykinin receptor
Weight
43204 MW
Superfamily
G-protein coupled receptor 1 family
Alternative Names
NF-kappa-B inhibitor-like protein 1;Inhibitor of kappa B-like protein;I-kappa-B-like protein;IkappaBL;Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor-like 1;NFKBIL1;IKBL; BDKRB1 B1BKR, B1R, BKB1R, BKR1, BRADYB1 bradykinin receptor B1 B1 bradykinin receptor|BK-1 receptor|bradykinin B1 receptor|bradykinin receptor 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Bdkrb1, check out the Bdkrb1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Bdkrb1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Bradykinin Receptor B1 Bdkrb1 Antibody (A05724)
Hello CJ!
No publications found for A05724
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Bradykinin Receptor B1 Bdkrb1 Antibody?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-Bradykinin Receptor B1 Bdkrb1 Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
6 Customer Q&As for Anti-Bradykinin Receptor B1 Bdkrb1 Antibody
Question
Which type of tissue was used in the western blot image shown on data sheet for A05724? Do you have this antibody in different host species other than Rabbit?
Verified customer
Asked: 2021-02-17
Answer
For the Anti-Bradykinin Receptor B1 Bdkrb1 Antibody (A05724), western blot analysis: MW marker (1) and 25µg rat heart extract (2) probed with Bradykinin Receptor (B1) pAb at 0.75µg/ml. Unfortunately, we do not have another host species.
Boster Scientific Support
Answered: 2021-02-17
Question
Have you tested A05724 using knockout mouse tissues?
Verified customer
Asked: 2021-02-17
Answer
Unfortunately, we have not tested the Anti-Bradykinin Receptor B1 Bdkrb1 Antibody (A05724) using knockout mouse tissues. This has been used for ELISA, IHC, WB.
Boster Scientific Support
Answered: 2021-02-18
Question
We are currently using anti-Bradykinin Receptor B1 antibody A05724 for mouse tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says mouse, rat. Is it likely that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2020-03-25
Answer
The anti-Bradykinin Receptor B1 antibody (A05724) has not been tested for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-03-25
Question
My question regarding product A05724, anti-Bradykinin Receptor B1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-08-16
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A05724 anti-Bradykinin Receptor B1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-08-16
Question
Is there a BSA free version of anti-Bradykinin Receptor B1 antibody A05724 available?
G. Johnson
Verified customer
Asked: 2019-05-07
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Bradykinin Receptor B1 antibody A05724 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-05-07
Question
I see that the anti-Bradykinin Receptor B1 antibody A05724 works with ELISA, what is the protocol used to produce the result images on the product page?
K. Rodriguez
Verified customer
Asked: 2014-03-12
Answer
You can find protocols for ELISA on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2014-03-12
