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Product Info Summary
| SKU: | A30599 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC-P, WB, IF (paraffin) |
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Product info
Product Name
Anti-BORG1 CDC42EP2 Antibody
SKU/Catalog Number
A30599
Size
100ul
Form
Liquid
Description
Boster Bio Anti-BORG1 CDC42EP2 Antibody (Catalog# A30599). Tested in WB, IHC-P, IF (paraffin), ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-BORG1 CDC42EP2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A30599)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human BORG1. Immunogen sequence location: 10-59
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A30599 is reactive to CDC42EP2 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
72 kDa
Calculated molecular weight
22484 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A30599 is guaranteed for ELISA, IHC-P, WB, IF (paraffin) Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western Blot, 1:500 - 1:2000
Immunohistochemistry, 1:100 - 1:300
ELISA, 1:40000
Not yet tested in other applications.
Validation Images & Assay Conditions
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Western blot analysis of lysates from K562 cells, using BORG1 Antibody. The lane on the right is blocked with the synthesized peptide.
Protein Target Info & Infographic
Gene/Protein Information For CDC42EP2 (Source: Uniprot.org, NCBI)
Gene Name
CDC42EP2
Full Name
Cdc42 effector protein 2
Weight
22484 MW
Superfamily
BORG/CEP family
Alternative Names
CDC42EP2;BORG1;CEP2;Cdc42 effector protein 2;Binder of Rho GTPases 1 CDC42EP2 BORG1, CEP2 CDC42 effector protein 2 cdc42 effector protein 2|CDC42 effector protein (Rho GTPase binding) 2|CRIB-containing BOGR1 protein|binder of Rho GTPases 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CDC42EP2, check out the CDC42EP2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CDC42EP2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-BORG1 CDC42EP2 Antibody (A30599)
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No publications found for A30599
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-BORG1 CDC42EP2 Antibody
Question
I have a question about product A30599, anti-BORG1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-04-01
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A30599 anti-BORG1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-04-01
Question
Is this A30599 anti-BORG1 antibody reactive to the isotypes of CDC42EP2?
Verified Customer
Verified customer
Asked: 2019-10-18
Answer
The immunogen of A30599 anti-BORG1 antibody is A peptide derived from human BORG1. Immunogen sequence location: 10-59. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-10-18
Question
We are currently using anti-BORG1 antibody A30599 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2018-10-05
Answer
The anti-BORG1 antibody (A30599) has not been tested for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-10-05
Question
Would anti-BORG1 antibody A30599 work for WB with lung pancreas?
E. Zhang
Verified customer
Asked: 2016-05-12
Answer
According to the expression profile of lung pancreas, CDC42EP2 is highly expressed in lung pancreas. So, it is likely that anti-BORG1 antibody A30599 will work for WB with lung pancreas.
Boster Scientific Support
Answered: 2016-05-12
Question
Does anti-BORG1 antibody A30599 work on bovine WB with brain?
G. Moore
Verified customer
Asked: 2016-05-06
Answer
Our lab technicians have not validated anti-BORG1 antibody A30599 on bovine. You can run a BLAST between bovine and the immunogen sequence of anti-BORG1 antibody A30599 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated bovine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in bovine brain in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2016-05-06
Question
Will A30599 anti-BORG1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
K. Johnson
Verified customer
Asked: 2016-03-25
Answer
As indicated on the product datasheet, A30599 anti-BORG1 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2016-03-25
