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Product Info Summary
| SKU: | M01469 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-BNIP3 Rabbit Monoclonal Antibody
SKU/Catalog Number
M01469
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-BNIP3 Rabbit Monoclonal Antibody catalog # M01469. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-BNIP3 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01469)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AAEI-2
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human BNIP3
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01469 is reactive to BNIP3 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
314 kDa
Calculated molecular weight
21541 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01469 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
Positive Control
IHC: Human pancreatic cancer tissue, Human placenta tissue, Human thyroid cancer tissue, human kidney tissue
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
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Western blot analysis of BNIP3 expression in (1) Jurkat cell lysate; (2) Mouse spleen lysate.
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Immunohistochemical analysis of paraffin-embedded Human pancreatic cancer, using the Antibody at 1:100 dilution.
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Immunohistochemical analysis of paraffin-embedded Human placenta, using the Antibody at 1:100 dilution.
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Immunohistochemical analysis of paraffin-embedded Human thyroid cancer, using the Antibody at 1:100 dilution.
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Immunohistochemical analysis of paraffin-embedded human kidney, using BNIP3 Antibody.
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Immunofluorescent analysis using the Antibody at 1:500 dilution.
Protein Target Info & Infographic
Gene/Protein Information For BNIP3 (Source: Uniprot.org, NCBI)
Gene Name
BNIP3
Full Name
BCL2/adenovirus E1B 19 kDa protein-interacting protein 3
Weight
21541 MW
Superfamily
NIP3 family
Alternative Names
BCL2/adenovirus E1B 19 kDa protein-interacting protein 3;BNIP3;NIP3; BNIP3 NIP3 BCL2 interacting protein 3 BCL2/adenovirus E1B 19 kDa protein-interacting protein 3|BCL2/adenovirus E1B 19kDa interacting protein 3|BCL2/adenovirus E1B interacting protein 3|nineteen kD interacting protein-3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on BNIP3, check out the BNIP3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for BNIP3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-BNIP3 Rabbit Monoclonal Antibody (M01469)
Hello CJ!
M01469 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Mutant HTT (huntingtin) impairs mitophagy in a cellular model of Huntington disease
Du Y, Li J, Xu T, Zhou DD, Zhang L, Wang X. Oncotarget. 2017 Jun 22;8(37):61510-61527. doi: 10.18632/oncotarget.18604. eCollection 2017 Sep 22. MicroRNA-145 induces apoptosis of glioma cells by targeting BNIP3 and Notch signaling
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Customer Reviews
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Customer Q&As
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3 Customer Q&As for Anti-BNIP3 Rabbit Monoclonal Antibody
Question
Is a blocking peptide available for product anti-BNIP3 Rabbit Monoclonal antibody (M01469)?
Verified Customer
Verified customer
Asked: 2020-03-20
Answer
We do provide the blocking peptide for product anti-BNIP3 Rabbit Monoclonal antibody (M01469). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-03-20
Question
I was wanting to use your anti-BNIP3 Rabbit Monoclonal antibody for WB for human cervix carcinoma on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human cervix carcinoma identification?
Verified Customer
Verified customer
Asked: 2019-01-24
Answer
As indicated on the product datasheet, M01469 anti-BNIP3 Rabbit Monoclonal antibody has been tested for IF, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human cervix carcinoma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-01-24
Question
Would M01469 anti-BNIP3 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
P. Thomas
Verified customer
Asked: 2015-03-05
Answer
You can see on the product datasheet, M01469 anti-BNIP3 Rabbit Monoclonal antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2015-03-05
