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Product Info Summary
| SKU: | M13425 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | ELISA, Flow Cytometry, IF, IHC, WB |
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Product info
Product Name
Anti-Bmi-1 COMMD3 Monoclonal Antibody
SKU/Catalog Number
M13425
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Bmi-1 COMMD3 Monoclonal Antibody catalog # M13425. Tested in ELISA, Flow Cytometry, IF, IHC, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Bmi-1 COMMD3 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M13425)
Host
Mouse
Contents
Ascitic fluid containing 0.03% sodium azide.
Clonality
Monoclonal
Clone Number
3E3
Isotype
IgG
Immunogen
Purified recombinant fragment of human Bmi-1 expressed in E. Coli.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M13425 is reactive to COMMD3 in Human
Reconstitution
Calculated molecular weight
22151 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M13425 is guaranteed for ELISA, Flow Cytometry, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:200-1:1000
IF 1:200-1:1000
FC 1:200-1:400
ELISA 1:10000
Validation Images & Assay Conditions
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Figure 5. ELISA validation of COMMD3 using Anti-Bmi-1 COMMD3 Monoclonal Antibody (M13425).
ELISA analysis of Bmi-1 antibody.
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Figure 4. Flow Cytometry validation of COMMD3 using Anti-Bmi-1 COMMD3 Monoclonal Antibody (M13425).
Flow cytometric (FCM) analysis of NIH/3T3 cells using Bmi-1 Monoclonal Antibody (green) and negative control (red).
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Figure 3. Immunofluorescent staining data of COMMD3 using Anti-Bmi-1 COMMD3 Monoclonal Antibody (M13425).
Immunofluorescence (IF) analysis of HeLa cells using Bmi-1 Monoclonal Antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
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Figure 2. Immunohistochemistry validation of COMMD3 using Anti-Bmi-1 COMMD3 Monoclonal Antibody (M13425).
Immunohistochemistry (IHC) analysis of paraffin-embedded cervical cancer tissues with DAB staining using Bmi-1 Monoclonal Antibody.For more protocol information of IHC
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Figure 1. Western blotting validation for Anti-Bmi-1 COMMD3 Monoclonal Antibody M13425
Western Blot (WB) analysis using Bmi-1 Monoclonal Antibody against HEK293 (1) and BMI1-hIgGFc transfected HEK293 (2) cell lysate.Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For COMMD3 (Source: Uniprot.org, NCBI)
Gene Name
COMMD3
Full Name
COMM domain-containing protein 3
Weight
22151 MW
Alternative Names
COMM domain-containing protein 3;Protein Bup;Protein PIL;COMMD3;BUP, C10orf8;
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on COMMD3, check out the COMMD3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for COMMD3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Bmi-1 COMMD3 Monoclonal Antibody (M13425)
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No publications found for M13425
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