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Product Info Summary
| SKU: | A02090-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IHC, WB |
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Product info
Product Name
Anti-Biglycan/BGN Antibody Picoband®
SKU/Catalog Number
A02090-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Biglycan/BGN Antibody Picoband® catalog # A02090-2. Tested in WB, IHC, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-Biglycan/BGN Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02090-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human Biglycan/BGN, identical to the related mouse and rat sequences.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A02090-2 is reactive to BGN in Human, Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
48 kDa
Calculated molecular weight
42 kDa
Background of Biglycan
This gene encodes a member of the small leucine-rich proteoglycan (SLRP) family of proteins. The encoded preproprotein is proteolytically processed to generate the mature protein, which plays a role in bone growth, muscle development and regeneration, and collagen fibril assembly in multiple tissues. This protein may also regulate inflammation and innate immunity. Additionally, the encoded protein may contribute to atherosclerosis and aortic valve stenosis in human patients. This gene and the related gene decorin are thought to be the result of a gene duplication.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02090-2 is guaranteed for Flow Cytometry, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Immunohistochemistry, 2-5 μg/ml, Human, Rat
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
Positive Control
WB: human COLO320 whole cell, human Hela whole cell, human HepG2 whole cell, human HUVEC whole cell, rat skeletal muscle tissue, rat L6 whole cell, mouse skeletal muscle tissue, mouse C2C12 whole cell
IHC: human breast cancer tissue, rat knee tissue
FCM: Caco-2 cells
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Biglycan/BGN using anti-Biglycan/BGN antibody (A02090-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human COLO320 whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: human HUVEC whole cell lysates,
Lane 5: rat skeletal muscle tissue lysates,
Lane 6: rat L6 whole cell lysates,
Lane 7: mouse skeletal muscle tissue lysates,
Lane 8: mouse C2C12 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Biglycan/BGN antigen affinity purified polyclonal antibody (Catalog # A02090-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Biglycan/BGN at approximately 48 kDa. The expected band size for Biglycan/BGN is at 42 kDa.
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Figure 2. IHC analysis of Biglycan/BGN using anti-Biglycan/BGN antibody (A02090-2).
Biglycan/BGN was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Biglycan/BGN Antibody (A02090-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Figure 3. IHC analysis of Biglycan/BGN using anti-Biglycan/BGN antibody (A02090-2).
Biglycan/BGN was detected in a paraffin-embedded section of rat knee tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Biglycan/BGN Antibody (A02090-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Figure 4. Flow Cytometry analysis of Caco-2 cells using anti-Biglycan/BGN antibody (A02090-2).
Overlay histogram showing Caco-2 cells stained with A02090-2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-Biglycan/BGN Antibody (A02090-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For BGN (Source: Uniprot.org, NCBI)
Gene Name
BGN
Full Name
Biglycan
Weight
42 kDa
Superfamily
small leucine-rich proteoglycan (SLRP) family
Alternative Names
BGN; Biglycan; Bone/cartilage proteoglycan I; bone/cartilage proteoglycan-I; dermatan sulphate proteoglycan I; DSPG1; PGI; PG-S1; SLRR1A; SLRR1Abiglycan proteoglycan; small leucine-rich protein 1A BGN DSPG1, MRLS, PG-S1, PGI, SEMDX, SLRR1A biglycan biglycan|bone/cartilage proteoglycan-I|dermatan sulphate proteoglycan I|small leucine-rich protein 1A
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on BGN, check out the BGN Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for BGN: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Biglycan/BGN Antibody Picoband® (A02090-2)
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