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Product Info Summary
| SKU: | A10479 |
|---|---|
| Size: | 50 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IP, IHC, WB |
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Product info
Product Name
Anti-BFAR (BAR) Antibody
SKU/Catalog Number
A10479
Size
50 µl
Form
Liquid
Description
Boster Bio Anti-BFAR (BAR) Antibody (Catalog# A10479). Tested in IP, IHC, WB application(s). This antibody reacts with Human.
Storage & Handling
Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.
Cite This Product
Anti-BFAR (BAR) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10479)
Host
Rabbit
Contents
50 µl sera
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide of BFAR (BAR) protein (amino acids 3-21 EPQKSYVNTMDLERDEPLK)
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A10479 is reactive to BFAR in Human
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
52738 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A10479 is guaranteed for IP, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000-1:2000
IHC (paraffin): 1:1000-1:5000
IHC (frozen): Users should optimize
IP: 1:50-1:200
Validation Images & Assay Conditions
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Fig:1 Western blot analysis of BAR in normal prostate and prostate carcinoma tissue lysates. 25 ug protein was loaded per lane. Tissue lysates from 15 different prostate carcinoma patients show variable expression of BAR with respect to banding patterns and amount of BAR expression. Major BAR bands typically migrate as a single band or as a doublet. These bands are typically observed at ~50-54 kDa. This is higher than the predicted molecular weight from the 450 amino acid BAR sequence, and may represent phosphorylation or other post-translational modifications. N = tissue lysate from normal prostate.
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Fig:2 Formalin-fixed, paraffin-embedded human prostate carcinoma tissue array stained for BAR expression using A10479 at 1:2000. Hematoxylin-eosin counterstain. Variable BAR expression is seen between patient samples
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Fig:3 Formalin-fixed, paraffin-embedded tissue sections stained for BAR expression using A10479 at 1:2000. A. Two cores from a human gliobastoma tissue microarray: 1 = fibrillary astrocytoma (grade I), and 2 = anaplastic glioma (grade III). B. Higher magnification from the fibrillary astrocytoma (shown in A). C. Higher magnification from the anaplastic glioma (shown in A). D. Normal human brain striatum with positive medium spiny neurons, the major neuronal cell type of the striatum. Hematoxylin-eosin counterstain.
Protein Target Info & Infographic
Gene/Protein Information For BFAR (Source: Uniprot.org, NCBI)
Gene Name
BFAR
Full Name
Bifunctional apoptosis regulator
Weight
52738 MW
Alternative Names
Bifunctional apoptosis regulator;RING finger protein 47;BFAR;BAR, RNF47; BFAR BAR, RNF47 bifunctional apoptosis regulator bifunctional apoptosis regulator|RING finger protein 47|bifunctional apoptosis inhibitor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on BFAR, check out the BFAR Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for BFAR: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-BFAR (BAR) Antibody (A10479)
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No publications found for A10479
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Customer Q&As
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1 Customer Q&As for Anti-BFAR (BAR) Antibody
Question
We are currently using anti-BFAR (BAR) antibody A10479 for human tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2018-02-01
Answer
The anti-BFAR (BAR) antibody (A10479) has not been tested for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-02-01
