Product Info Summary
SKU: | A06868-1 |
---|---|
Size: | 100μl |
Reactive Species: | Mouse, Rat |
Host: | Mouse |
Application: | IP, IHC, WB |
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Product info
Product Name
Anti-beta II tubulin TUBB2A Monoclonal Antibody
View all beta II Tubulin A Antibodies
SKU/Catalog Number
A06868-1
Size
100μl
Form
Liquid
Description
Boster Bio Anti-beta II tubulin TUBB2A Monoclonal Antibody catalog # A06868-1. Tested in IP, IHC, WB applications. This antibody reacts with Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-beta II tubulin TUBB2A Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06868-1)
Host
Mouse
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Monoclonal
Clone Number
Mix
Isotype
IgG1
Immunogen
Synthetic Peptide of β II tubulin
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A06868-1 is reactive to TUBB2A in Mouse, Rat
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
49907 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A06868-1 is guaranteed for IP, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:50000-100000
IP, 0.180555555555556
Validation Images & Assay Conditions
Click image to see more details
Western Blot (WB) analysis of 1) Mouse Brain tissue, 2) Rat Brain tissue, diluted at 1:100000.
Click image to see more details
Immunoprecipitation (IP) analysis: 1) Input: Mouse Brain Tissue Lysate.
Protein Target Info & Infographic
Gene/Protein Information For TUBB2A (Source: Uniprot.org, NCBI)
Gene Name
TUBB2A
Full Name
Tubulin beta-2A chain
Weight
49907 MW
Superfamily
tubulin family
Alternative Names
Tubulin beta-2A chain;Tubulin beta class IIa;TUBB2A;TUBB2; TUBB2A CDCBM5, TUBB, TUBB2 tubulin beta 2A class IIa tubulin beta-2A chain|class IIa beta-tubulin|tubulin, beta 2A|tubulin, beta polypeptide 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TUBB2A, check out the TUBB2A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TUBB2A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-beta II tubulin TUBB2A Monoclonal Antibody (A06868-1)
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No publications found for A06868-1
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Customer Q&As
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4 Customer Q&As for Anti-beta II tubulin TUBB2A Monoclonal Antibody
Question
We are currently using anti- beta II tubulin Monoclonal antibody A06868-1 for mouse tissue, and we are content with the IP results. The species of reactivity given in the datasheet says mouse, rat. Is it possible that the antibody can work on primate tissues as well?
J. Lewis
Verified customer
Asked: 2019-06-11
Answer
The anti- beta II tubulin Monoclonal antibody (A06868-1) has not been validated for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-06-11
Question
Would A06868-1 anti- beta II tubulin Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
C. Zhao
Verified customer
Asked: 2018-12-17
Answer
As indicated on the product datasheet, A06868-1 anti- beta II tubulin Monoclonal antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-12-17
Question
I would like to test anti- beta II tubulin Monoclonal antibody A06868-1 on rat brain for research purposes, then I may be interested in using anti- beta II tubulin Monoclonal antibody A06868-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
A. Gonzalez
Verified customer
Asked: 2014-05-02
Answer
The products we sell, including anti- beta II tubulin Monoclonal antibody A06868-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2014-05-02
Question
I have a question about product A06868-1, anti- beta II tubulin Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
G. Mitchell
Verified customer
Asked: 2013-03-13
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A06868-1 anti- beta II tubulin Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2013-03-13