Anti-beta-hCG Purified CGB3 Monoclonal Antibody

Product Name

Anti-beta-hCG Purified CGB3 Monoclonal Antibody

View all CGB3 Antibodies

SKU/Catalog Number

M13820

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-beta-hCG Purified CGB3 Monoclonal Antibody (Catalog# M13820). Tested in ICC, ELISA application(s). This antibody reacts with Human.

Storage & Handling

Store at 2-8°C. Do not freeze.

Cite This Product

Anti-beta-hCG Purified CGB3 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M13820)

Host

Mouse

Contents

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Clonality

Monoclonal

Clone Number

HCG-61

Isotype

Mouse IgG2b

Immunogen

Human chorionic gonadotropin. The antibody HCG-61 reacts with beta-subunit of human chorionic gonadotropin, a 40 kDa hormone. hCG belongs to the glycoprotein hormone family together with lutropin (LH), follitropin (FSH) and thyrotropin (TSH). hCG is synthesised by trophoblastic cells of the placenta during pregnancy and stimulates the growth of corpus luteum. The HCG-61 antibody reacts with association constant 5.1 x 10¹⁰ l/mol.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Following cross-reactivity (%) was determined by classic double-antibody RIA using unlabelled hormones: beta-hCG (77), alpha-hCG (1.3), hLH (0.86), hFSH (< 0.5), hTSH (< 0.5).

Reactive Species

M13820 is reactive to CGB in Human

Reconstitution

Observed Molecular Weight

42 kDa

Calculated molecular weight

17739 MW

Background of CGB3

Choriogonadotropin (hCG) is a protein of the molecular weight about 40 kDa. It belongs to the glycoprotein hormone family together with lutropin (LH), follitropin (FSH) and thyrotropin (TSH). Choriogonadotropin is synthesised by trophoblastic cells of the placenta during pregnancy and stimulates the growth of corpus luteum. The other hormones are produced by anterior pituitary gland. All these hormones are structurally related, being composed of two noncovalently associated subunits alpha and beta.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M13820 is guaranteed for ELISA, ICC Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunocytochemistry: 10 μg/ml; cell culture fixed with 4% paraformaldehyde, permeabilized with 0.1% Triton-X100.
Western blotting: 1 μg/ml; positive control: murine brain lysate (red. Laemmli buffer).

Validation Images & Assay Conditions

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Gene/Protein Information For CGB (Source: Uniprot.org, NCBI)

Alternative Names

Choriogonadotropin beta, CG-beta CGB3 CGB, CGB5, CGB7, CGB8, LHB, hCGB chorionic gonadotropin subunit beta 3 choriogonadotropin subunit beta 3|CG-beta|chorionic gonadotrophin chain beta|chorionic gonadotropin beta 3 subunit|chorionic gonadotropin beta chain|chorionic gonadotropin beta subunit 3|chorionic gonadotropin chain beta|chorionic gonadotropin, beta polypeptide|luteinizing hormone beta subunit

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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