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Product Info Summary
| SKU: | PA1450 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-beta Defensin 1/DEFB1 Antibody
SKU/Catalog Number
PA1450
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-beta Defensin 1/DEFB1 Antibody catalog # PA1450. Tested in IHC, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-beta Defensin 1/DEFB1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1450)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human beta Defensin 1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1450 is reactive to DEFB1 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
7 kDa
Calculated molecular weight
7420 MW
Background of DEFB1
DEFB1,Beta-defensin 1, is a protein that in humans is encoded by the DEFB1 gene. DEFB1 belongs to the defensin family. Defensins form a family of microbicidal and cytotoxic peptides made by neutrophils. The DEFB1 gene spans more than 7 kb and includes a large 6,962-bp intron. By 2-color fluorescence in situ hybridization on both metaphase chromosomes and released chromatin fiber, the DEFB1gene is mapped to 8p23.2-p23.1, within 100 to 150 kb of the gene for human neutrophil defensin-alpha-1. DEFB1may augment neonatal host defenses through antimicrobial effects or prime the adaptive immune system at mucosal surfaces.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1450 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: COLO320 Whole Cell
IHC: Lung Cancer Tissue
Validation Images & Assay Conditions
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Anti-beta Defensin 1 antibody, PA1450, IHC(P)
IHC(P): Lung Cancer Tissue
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Anti-beta Defensin 1 antibody, PA1450,Western blotting
All lanes: Anti Defensin 1 (PA1450) at 0.5ug/ml
WB : COLO320 Whole Cell Lysate at 40ug
Predicted bind size: 7KD
Observed bind size: 7KD
Protein Target Info & Infographic
Gene/Protein Information For DEFB1 (Source: Uniprot.org, NCBI)
Gene Name
DEFB1
Full Name
Beta-defensin 1
Weight
7420 MW
Superfamily
beta-defensin family
Alternative Names
Beta-defensin 1;BD-1;hBD-1;Defensin, beta 1;DEFB1;BD1, HBD1; DEFB1 BD1, DEFB-101, HBD1, DEFB1 defensin beta 1 beta-defensin 1|BD-1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on DEFB1, check out the DEFB1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for DEFB1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-beta Defensin 1/DEFB1 Antibody (PA1450)
Hello CJ!
PA1450 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
An investigation of human beta-defensins and cathelicidin expression in patients with pterygium
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Customer Q&As
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1 Customer Q&As for Anti-beta Defensin 1/DEFB1 Antibody
Question
We are currently using anti-beta Defensin 1/DEFB1 antibody PA1450 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2019-08-15
Answer
The anti-beta Defensin 1/DEFB1 antibody (PA1450) has not been validated for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-08-15
