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Product Info Summary
| SKU: | A09277 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse |
| Host: | Mouse |
| Application: | IHC, WB |
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Product info
Product Name
Anti-BDH1 Monoclonal Antibody
SKU/Catalog Number
A09277
Size
100μl
Form
Liquid
Description
Boster Bio Anti-BDH1 Monoclonal Antibody catalog # A09277. Tested in IHC, WB applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-BDH1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09277)
Host
Mouse
Contents
Ascitic fluid containing 0.03% sodium azide.
Clonality
Monoclonal
Clone Number
1A5
Isotype
IgG
Immunogen
Purified recombinant fragment of human BDH1 expressed in E. Coli.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A09277 is reactive to BDH1 in Human, Mouse
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
38157 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A09277 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
IHC, 1:200-1:1000
Validation Images & Assay Conditions
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Immunohistochemistry (IHC) analysis of paraffin-embedded Human Liver cancer (left) and colorectal cancer tissues (right) with DAB staining using BDH1 Monoclonal antibody.
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Western Blot (WB) analysis using BDH1 Monoclonal antibody against HepG2 (1) and NIH/3T3 (2) cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For BDH1 (Source: Uniprot.org, NCBI)
Gene Name
BDH1
Full Name
D-beta-hydroxybutyrate dehydrogenase, mitochondrial
Weight
38157 MW
Superfamily
short-chain dehydrogenases/reductases (SDR) family
Alternative Names
D-beta-hydroxybutyrate dehydrogenase, mitochondrial ;1.1.1.30 ;3-hydroxybutyrate dehydrogenase ;BDH ;Short chain dehydrogenase/reductase family 9C member 1;BDH1 ;BDH , SDR9C1; BDH1 BDH, SDR9C1 3-hydroxybutyrate dehydrogenase 1 D-beta-hydroxybutyrate dehydrogenase, mitochondrial|(R)-3-hydroxybutyrate dehydrogenase|3-hydroxybutyrate dehydrogenase (heart, mitochondrial)|3-hydroxybutyrate dehydrogenase, type 1|epididymis secretory sperm binding protein|short chain dehydrogenase/reductase family 9C member 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on BDH1, check out the BDH1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for BDH1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-BDH1 Monoclonal Antibody (A09277)
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No publications found for A09277
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-BDH1 Monoclonal Antibody
Question
Would A09277 anti-BDH1 Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-04-22
Answer
It shows on the product datasheet, A09277 anti-BDH1 Monoclonal antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-04-22
Question
We are currently using anti-BDH1 Monoclonal antibody A09277 for mouse tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human, mouse. Is it possible that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2019-10-30
Answer
The anti-BDH1 Monoclonal antibody (A09277) has not been validated for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-10-30
Question
Will anti-BDH1 Monoclonal antibody A09277 work on primate IHC with lung ovary?
H. Carter
Verified customer
Asked: 2019-09-23
Answer
Our lab technicians have not validated anti-BDH1 Monoclonal antibody A09277 on primate. You can run a BLAST between primate and the immunogen sequence of anti-BDH1 Monoclonal antibody A09277 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate lung ovary in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-09-23
Question
Is a blocking peptide available for product anti-BDH1 Monoclonal antibody (A09277)?
Verified Customer
Verified customer
Asked: 2019-07-11
Answer
We do provide the blocking peptide for product anti-BDH1 Monoclonal antibody (A09277). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-07-11
Question
I see that the anti-BDH1 Monoclonal antibody A09277 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-11-07
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-11-07
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for right lobe of liver using anti-BDH1 Monoclonal antibody A09277. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-09-07
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-09-07
Question
Will anti-BDH1 Monoclonal antibody A09277 work for WB with right lobe of liver?
B. Li
Verified customer
Asked: 2016-09-30
Answer
According to the expression profile of right lobe of liver, BDH1 is highly expressed in right lobe of liver. So, it is likely that anti-BDH1 Monoclonal antibody A09277 will work for WB with right lobe of liver.
Boster Scientific Support
Answered: 2016-09-30
