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Product Info Summary
| SKU: | A02432 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-Bcl-2-modifying factor Bmf Antibody
SKU/Catalog Number
A02432
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Bcl-2-modifying factor Bmf Antibody (Catalog # A02432). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human.
Storage & Handling
Bmf antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-Bcl-2-modifying factor Bmf Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02432)
Host
Rabbit
Contents
Bmf Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Bmf antibody was raised with a synthetic peptide corresponding to 14 amino acids near the carboxy terminus of human Bmf. The immunogen is located within the last 50 amino acids of Bmf.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
At least four isoforms are known to exist; this antibody will only detect the largest isoform. PHLPP1 antibody is predicted to not cross react with PHLPP2.
Reactive Species
A02432 is reactive to BMF in Human
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
20508 MW
Background of BMF
Apoptosis is related to many diseases and development. Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bcl-2 homology 3 (BH3) domain is a potent death domain. BH3-only proteins, including Bad, Bid, Bik, Hrk, Bim, Noxa, and PUMA, form a growing subclass of the Bcl-2 family. A novel BH3-only protein was recently identified in human and mouse and designated Bmf (for Bcl-2-modifing factor). The BH3 domain in Bmf is required both for binding to Bcl-2 proteins and for triggering apoptosis. In healthy cells, Bmf associates with the dynein light chain 2 (DLC2) component of the myosin V motors and is sequestered by the cell's actin cytoskeleton. Disruption of the actin cytoskeleton, either by depolymerization of actin filaments or by detachment of cells from the extracellular matrix, triggers release and activation of Bmf, initiating the downstream apoptotic program. Bmf is constitutively expressed in many tissues.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02432 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Bmf antibody can be used for detection of Bmf by Western blot at 2.5 and 5 μg/mL. A band at approximately 25 kDa can be detected. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 10 μg/mL.
Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of Bmf expression in HepG2 cell lysate with Bmf antibody at (A) 2.5 and (B) 5 μg/mL.
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Immunofluorescence of Bmf in human kidney tissue with Bmf antibody at 10 μg/mL.
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Immunofluorescence of Bmf in Human Kidney cells with Bmf antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For BMF (Source: Uniprot.org, NCBI)
Gene Name
BMF
Full Name
Bcl-2-modifying factor
Weight
20508 MW
Superfamily
Bcl-2 family
Alternative Names
Bcl-2-modifying factor BMF Bcl2 modifying factor bcl-2-modifying factor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on BMF, check out the BMF Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for BMF: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Bcl-2-modifying factor Bmf Antibody (A02432)
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