Product Info Summary
SKU: | A11264 |
---|---|
Size: | 100 µl |
Reactive Species: | Human |
Host: | Rabbit |
Application: | IHC |
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Product info
Product Name
Anti-ATP5G2 Antibody
SKU/Catalog Number
A11264
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-ATP5G2 Antibody (Catalog # A11264). Tested in IHC applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ATP5G2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A11264)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human ATP5G2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A11264 is reactive to ATP5G2 in Human
Reconstitution
Calculated molecular weight
14637 MW
Background of ATP5MC2
Mitochondrial membrane ATP synthase (F1F0 ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F1 - containing the extramembraneous catalytic core and F0 - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F1 is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F0 domain. A homomeric c-ring of probably 10 subunits is part of the complex rotary element. HAMAP-Rule MF_01396
Dyer M.R., Biochem. J. 293:51-64(1993).
Higuti T., Biochim. Biophys. Acta 1173:87-90(1993).
Farrell L.B., Biochem. Biophys. Res. Commun. 144:1257-1264(1987).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A11264 is guaranteed for IHC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry: 1:50~1:100
Validation Images & Assay Conditions
Click image to see more details
Immunohistochemistry analysis of paraffin-embedded human pancreas tissue using ATP5G2 antibody A11264.
Protein Target Info & Infographic
Gene/Protein Information For ATP5G2 (Source: Uniprot.org, NCBI)
Gene Name
ATP5G2
Full Name
ATP synthase F(0) complex subunit C2, mitochondrial
Weight
14637 MW
Superfamily
ATPase C chain family
Alternative Names
ATP synthase F (0) complex subunit C2, mitochondrial;ATP synthase lipid-binding protein;ATP synthase proteolipid P2;ATP synthase proton-transporting mitochondrial F (0) complex subunit C2;ATPase protein 9;ATPase subunit c;ATP5G2;PSEC0033; ATP5MC2 ATP5A, ATP5G2 ATP synthase membrane subunit c locus 2 ATP synthase F(0) complex subunit C2, mitochondrial|ATP synthase c subunit|ATP synthase lipid-binding protein, mitochondrial|ATP synthase proteolipid P2|ATP synthase proton-transporting mitochondrial F(0) complex subunit C2|ATP synthase, H+ transporting, mitochondrial F0 complex, subunit C2 (subunit 9)|ATP synthase, H+ transporting, mitochondrial Fo complex subunit C2 (subunit 9)|ATPase protein 9|ATPase subunit C|dicyclohexylcarbodiimide (DCCD)-reactive proteolipid subunit
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ATP5G2, check out the ATP5G2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ATP5G2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ATP5G2 Antibody (A11264)
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No publications found for A11264
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Customer Q&As
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6 Customer Q&As for Anti-ATP5G2 Antibody
Question
Would A11264 anti-ATP5G2 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-04-29
Answer
It shows on the product datasheet, A11264 anti-ATP5G2 antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-04-29
Question
Is this A11264 anti-ATP5G2 antibody reactive to the isotypes of Atp5g2?
Verified Customer
Verified customer
Asked: 2020-04-17
Answer
The immunogen of A11264 anti-ATP5G2 antibody is Synthesized peptide derived from internal of human ATP5G2. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-04-17
Question
I am interested in to test anti-ATP5G2 antibody A11264 on human liver for research purposes, then I may be interested in using anti-ATP5G2 antibody A11264 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-08-09
Answer
The products we sell, including anti-ATP5G2 antibody A11264, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-08-09
Question
See attached the WB image, lot number and protocol we used for liver using anti-ATP5G2 antibody A11264. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-08-06
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-08-06
Question
I see that the anti-ATP5G2 antibody A11264 works with IHC, what is the protocol used to produce the result images on the product page?
O. Anderson
Verified customer
Asked: 2019-06-17
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-06-17
Question
We are currently using anti-ATP5G2 antibody A11264 for human tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2018-06-04
Answer
The anti-ATP5G2 antibody (A11264) has not been tested for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-06-04