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Product Info Summary
| SKU: | A02212-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-ASS1 Picoband® Antibody
View all Argininosuccinate Synthase Antibodies
SKU/Catalog Number
A02212-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-ASS1 Picoband® Antibody catalog # A02212-1. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ASS1 Picoband® Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02212-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human ASS1 recombinant protein (Position: S3-S365).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A02212-1 is reactive to ASS1 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
47 kDa
Calculated molecular weight
38160 MW
Background of Argininosuccinate Synthase
Argininosuccinate synthetase is an enzyme that in humans is encoded by the ASS1 gene. It is mapped to 9q34.11. The protein encoded by this gene catalyzes the penultimate step of the arginine biosynthetic pathway. There are approximately 10 to 14 copies of this gene including the pseudogenes scattered across the human genome, among which the one located on chromosome 9 appears to be the only functional gene for argininosuccinate synthetase. Mutations in the chromosome 9 copy of this gene cause citrullinemia. Two transcript variants encoding the same protein have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02212-1 is guaranteed for ELISA, Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.25μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human
ELISA, 0.1-0.5μg/ml, -
Positive Control
WB: human HELA whole cell, human HEK293 whole cell, human THP-1 whole cell, human MDA-MB-453 whole cell, human A431 whole cell, human HL-60 whole cell, human U87 whole cell, rat kidney tissue, rat liver tissue, mouse kidney tissue, mouse liver tissue
IHC: human Pancreatic Cancer tissue
ICC/IF: HEPG2 cell
FCM: THP-1 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of ASS1 using anti-ASS1 antibody (A02212-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human HELA whole cell lysates,
Lane 2: human HEK293 whole cell lysates,
Lane 3: human THP-1 whole cell lysates,
Lane 4: human MDA-MB-453 whole cell lysates,
Lane 5: human A431 whole cell lysates,
Lane 6: human HL-60 whole cell lysates,
Lane 7: human U87 whole cell lysates,
Lane 8: rat kidney tissue lysates,
Lane 9: rat liver tissue lysates,
Lane 10: mouse kidney tissue lysates,
Lane 11: mouse liver tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ASS1 antigen affinity purified polyclonal antibody (Catalog # A02212-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ASS1 at approximately 47KD. The expected band size for ASS1 is at 47KD.
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Figure 2. IHC analysis of ASS1 using anti-ASS1 antibody (A02212-1).
ASS1 was detected in paraffin-embedded section of human Pancreatic Cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ASS1 Antibody (A02212-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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Figure 3. IF analysis of ASS1 using anti-ASS1 antibody (A02212-1).
ASS1 was detected in immunocytochemical section of HEPG2 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-ASS1 Antibody (A02212-1) overnight at 4°C. DyLight®488 conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Figure 4. Flow Cytometry analysis of THP-1 cells using anti-ASS1 antibody (A02212-1).
Overlay histogram showing THP-1 cells stained with A02212-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ASS1 Antibody (A02212-1, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For ASS1 (Source: Uniprot.org, NCBI)
Gene Name
ASS1
Full Name
Argininosuccinate synthase
Weight
38160 MW
Superfamily
argininosuccinate synthase family
Alternative Names
Argininosuccinate synthase; Citrulline--aspartate ligase; ASS1; ASS ASS1 ASS, CTLN1 argininosuccinate synthase 1 argininosuccinate synthase|argininosuccinate synthetase 1|citrulline-aspartate ligase|epididymis secretory sperm binding protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ASS1, check out the ASS1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ASS1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ASS1 Picoband® Antibody (A02212-1)
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