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Product Info Summary
| SKU: | A03023 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC |
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Product info
Product Name
Anti-ASCL1/Mash1 Antibody
View all ASCL1/Mash1 Antibodies
SKU/Catalog Number
A03023
Size
100μl
Form
Liquid
Description
Boster Bio Anti-ASCL1/Mash1 Antibody catalog # A03023. Tested in ELISA, IHC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ASCL1/Mash1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03023)
Host
Rabbit
Contents
PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthetic peptide from human protein at AA range: 190-236
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A03023 is reactive to ASCL1 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
25454 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03023 is guaranteed for ELISA, IHC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IHC-P 1:50-200
ELISA 1:10000-20000
Validation Images & Assay Conditions
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Immunohistochemical analysis of paraffin-embedded human-lung, antibody was diluted at 1:200.
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Immunohistochemical analysis of paraffin-embedded human-brain, antibody was diluted at 1:200.
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Immunohistochemical analysis of paraffin-embedded human-lung, antibody was diluted at 1:200.
Protein Target Info & Infographic
Gene/Protein Information For ASCL1 (Source: Uniprot.org, NCBI)
Gene Name
ASCL1
Full Name
Achaete-scute homolog 1
Weight
25454 MW
Alternative Names
Achaete-scute homolog 1;ASH-1;hASH1;Class A basic helix-loop-helix protein 46;bHLHa46;ASCL1;ASH1, BHLHA46, HASH1; ASCL1 ASH1, HASH1, MASH1, bHLHa46 achaete-scute family bHLH transcription factor 1 achaete-scute homolog 1|ASH-1|achaete scute protein|achaete-scute complex homolog 1|achaete-scute complex-like 1|class A basic helix-loop-helix protein 46
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ASCL1, check out the ASCL1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ASCL1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ASCL1/Mash1 Antibody (A03023)
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Customer Q&As
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6 Customer Q&As for Anti-ASCL1/Mash1 Antibody
Question
We are currently using anti-ASCL1/Mash1 antibody A03023 for mouse tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2020-03-09
Answer
The anti-ASCL1/Mash1 antibody (A03023) has not been tested for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-03-09
Question
Would A03023 anti-ASCL1/Mash1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-02-28
Answer
As indicated on the product datasheet, A03023 anti-ASCL1/Mash1 antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-02-28
Question
Is a blocking peptide available for product anti-ASCL1/Mash1 antibody (A03023)?
Verified Customer
Verified customer
Asked: 2020-02-19
Answer
We do provide the blocking peptide for product anti-ASCL1/Mash1 antibody (A03023). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-02-19
Question
I would like to test anti-ASCL1/Mash1 antibody A03023 on rat brain for research purposes, then I may be interested in using anti-ASCL1/Mash1 antibody A03023 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-12-04
Answer
The products we sell, including anti-ASCL1/Mash1 antibody A03023, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-12-04
Question
I have a question about product A03023, anti-ASCL1/Mash1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-12-28
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A03023 anti-ASCL1/Mash1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-12-28
Question
Is there a BSA free version of anti-ASCL1/Mash1 antibody A03023 available?
Verified Customer
Verified customer
Asked: 2018-01-12
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-ASCL1/Mash1 antibody A03023 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-01-12
