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Product Info Summary
| SKU: | A01609-3 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Arsb Antibody Picoband®
View all Arylsulfatase B/ARSB Antibodies
SKU/Catalog Number
A01609-3
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Arsb Antibody Picoband® catalog # A01609-3. Tested in WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Arsb Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01609-3)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of mouse Arsb, which shares 88.2% and 94.1% amino acid (aa) sequence identity with human and rat Arsb, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01609-3 is reactive to Arsb in Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
66-70 kDa
Calculated molecular weight
59.687kDa
Background of Arylsulfatase B/ARSB
Arylsulfatase B encoded by this gene belongs to the sulfatase family. The arylsulfatase B homodimer hydrolyzes sulfate groups of N-Acetyl-D-galactosamine, chondriotin sulfate, and dermatan sulfate. The protein is targeted to the lysozyme. Mucopolysaccharidosis type VI is an autosomal recessive lysosomal storage disorder resulting from a deficiency of arylsulfatase B. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01609-3 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Mouse, Rat
Positive Control
WB: rat Rh35 whole cell, rat C6 whole cell, mouse HEPA1-6 whole cell, mouse Neuro-2a whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Arsb using anti-Arsb antibody (A01609-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat Rh35 whole cell lysates,
Lane 2: rat C6 whole cell lysates,
Lane 3: mouse HEPA1-6 whole cell lysates,
Lane 4: mouse Neuro-2a whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Arsb antigen affinity purified polyclonal antibody (Catalog # A01609-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Arsb at approximately 66-70KD. The expected band size for Arsb is at 60KD.
Protein Target Info & Infographic
Gene/Protein Information For Arsb (Source: Uniprot.org, NCBI)
Gene Name
Arsb
Full Name
Arylsulfatase B
Weight
59.687kDa
Superfamily
sulfatase family
Alternative Names
Ras-related protein Rab-27A; Rab-27; GTP-binding protein Ram; RAB27A; RAB27 ARSB ASB, G4S, MPS6 arylsulfatase B arylsulfatase B|N-acetylgalactosamine-4-sulfatase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Arsb, check out the Arsb Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Arsb: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Arsb Antibody Picoband® (A01609-3)
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