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Product Info Summary
| SKU: | A09689 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, WB |
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Product info
Product Name
Anti-ARFIP1/Arfaptin 1 Antibody
SKU/Catalog Number
A09689
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-ARFIP1/Arfaptin 1 Antibody (Catalog # A09689). Tested in WB, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ARFIP1/Arfaptin 1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09689)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human ARFIP1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A09689 is reactive to ARFIP1 in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
41738 MW
Background of ARFIP1
Putative target protein of ADP-ribosylation factor.
Kanoh H., J. Biol. Chem. 272:5421-5429(1997).
Premont R.T., Submitted (JAN-1999) to the EMBL/GenBank/DDBJ databases.
The MGC Project Team; Genome Res. 14:2121-2127(2004).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A09689 is guaranteed for IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Immunofluorescence: 1:100~1:500
Validation Images & Assay Conditions
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Western blot analysis of extracts from Jurkat cells, using ARFIP1 antibody A09689.
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Immunofluorescence analysis of A549 cells, using ARFIP1 antibody A09689.
Protein Target Info & Infographic
Gene/Protein Information For ARFIP1 (Source: Uniprot.org, NCBI)
Gene Name
ARFIP1
Full Name
Arfaptin-1
Weight
41738 MW
Alternative Names
Arfaptin-1;ADP-ribosylation factor-interacting protein 1;ARFIP1; ARFIP1 HSU52521 ADP ribosylation factor interacting protein 1 arfaptin-1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ARFIP1, check out the ARFIP1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ARFIP1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ARFIP1/Arfaptin 1 Antibody (A09689)
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-ARFIP1/Arfaptin 1 Antibody
Question
Does A09689 anti-ARFIP1/Arfaptin 1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-05-01
Answer
You can see on the product datasheet, A09689 anti-ARFIP1/Arfaptin 1 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-05-01
Question
I see that the anti-ARFIP1/Arfaptin 1 antibody A09689 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-12-26
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-12-26
Question
Is there a BSA free version of anti-ARFIP1/Arfaptin 1 antibody A09689 available?
Verified Customer
Verified customer
Asked: 2017-08-22
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-ARFIP1/Arfaptin 1 antibody A09689 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-08-22
Question
We are currently using anti-ARFIP1/Arfaptin 1 antibody A09689 for mouse tissue, and we are satisfied with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2017-06-08
Answer
The anti-ARFIP1/Arfaptin 1 antibody (A09689) has not been tested for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-06-08
Question
I was wanting to use your anti-ARFIP1/Arfaptin 1 antibody for WB for mouse liver on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse liver identification?
A. Carter
Verified customer
Asked: 2013-10-25
Answer
It shows on the product datasheet, A09689 anti-ARFIP1/Arfaptin 1 antibody has been validated for IF, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse liver in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2013-10-25
Question
See below the WB image, lot number and protocol we used for liver using anti-ARFIP1/Arfaptin 1 antibody A09689. Please let me know if you require anything else.
W. Huang
Verified customer
Asked: 2013-09-11
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2013-09-11
