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Product Info Summary
| SKU: | A04746 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-ARALAR SLC25A12 Antibody
SKU/Catalog Number
A04746
Size
100μl
Form
Liquid
Description
Boster Bio Anti-ARALAR SLC25A12 Antibody catalog # A04746. Tested in WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ARALAR SLC25A12 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04746)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human CMC1. AA range:391-440
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A04746 is reactive to SLC25A12 in Human
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
74762 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04746 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
Validation Images & Assay Conditions
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Western Blot (WB) analysis of HepG2 cells using ARALAR Polyclonal antibody.
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Western Blot (WB) analysis of specific cells using ARALAR Polyclonal antibody.
Protein Target Info & Infographic
Gene/Protein Information For SLC25A12 (Source: Uniprot.org, NCBI)
Gene Name
SLC25A12
Full Name
Calcium-binding mitochondrial carrier protein Aralar1
Weight
74762 MW
Superfamily
mitochondrial carrier (TC 2.A.29) family
Alternative Names
Calcium-binding mitochondrial carrier protein Aralar1;Mitochondrial aspartate glutamate carrier 1;Solute carrier family 25 member 12;SLC25A12;ARALAR1; SLC25A12 AGC1, ARALAR, DEE39, EIEE39 solute carrier family 25 member 12 calcium-binding mitochondrial carrier protein Aralar1|araceli hiperlarga|calcium binding mitochondrial carrier superfamily member Aralar1|mitochondrial aspartate glutamate carrier 1|solute carrier family 25 (aspartate/glutamate carrier), member 12|solute carrier family 25 (mitochondrial carrier, Aralar), member 12
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SLC25A12, check out the SLC25A12 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SLC25A12: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ARALAR SLC25A12 Antibody (A04746)
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No publications found for A04746
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Recommended Resources
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Customer Reviews
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Customer Q&As
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13 Customer Q&As for Anti-ARALAR SLC25A12 Antibody
Question
We are currently using anti-ARALAR antibody A04746 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2020-03-31
Answer
The anti-ARALAR antibody (A04746) has not been tested for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-03-31
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for lymph using anti-ARALAR antibody A04746. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-12-26
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-26
Question
Is there a BSA free version of anti-ARALAR antibody A04746 available?
Verified Customer
Verified customer
Asked: 2019-12-05
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-ARALAR antibody A04746 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-12-05
Question
Please see the WB image, lot number and protocol we used for lymph using anti-ARALAR antibody A04746. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-11-22
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-11-22
Question
Would anti-ARALAR antibody A04746 work on bovine WB with muscle of leg?
Verified Customer
Verified customer
Asked: 2019-09-17
Answer
Our lab technicians have not validated anti-ARALAR antibody A04746 on bovine. You can run a BLAST between bovine and the immunogen sequence of anti-ARALAR antibody A04746 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated bovine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in bovine muscle of leg in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-09-17
Question
Is a blocking peptide available for product anti-ARALAR antibody (A04746)?
Verified Customer
Verified customer
Asked: 2019-07-30
Answer
We do provide the blocking peptide for product anti-ARALAR antibody (A04746). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-07-30
Question
I was wanting to use your anti-ARALAR antibody for WB for human lymph on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human lymph identification?
Verified Customer
Verified customer
Asked: 2019-05-31
Answer
You can see on the product datasheet, A04746 anti-ARALAR antibody has been validated for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human lymph in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-05-31
Question
Is this A04746 anti-ARALAR antibody reactive to the isotypes of SLC25A12?
P. Collins
Verified customer
Asked: 2019-04-10
Answer
The immunogen of A04746 anti-ARALAR antibody is Synthesized peptide derived from the Internal region of human ARALAR. at AA rangle: 360-440. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-04-10
Question
Will A04746 anti-ARALAR antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-04-05
Answer
As indicated on the product datasheet, A04746 anti-ARALAR antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-04-05
Question
I see that the anti-ARALAR antibody A04746 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-06-01
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-06-01
Question
I have a question about product A04746, anti-ARALAR antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-04-10
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A04746 anti-ARALAR antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-04-10
Question
you antibody to test anti-ARALAR antibody A04746 on human lymph for research purposes, then I may be interested in using anti-ARALAR antibody A04746 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
C. Taylor
Verified customer
Asked: 2018-02-14
Answer
The products we sell, including anti-ARALAR antibody A04746, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-02-14
Question
Would anti-ARALAR antibody A04746 work for WB with lymph?
D. Moore
Verified customer
Asked: 2013-06-13
Answer
According to the expression profile of lymph, SLC25A12 is highly expressed in lymph. So, it is likely that anti-ARALAR antibody A04746 will work for WB with lymph.
Boster Scientific Support
Answered: 2013-06-13
