Product Info Summary
SKU: | PA1654-1 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-Amyloid-like protein 2 APLP2 Antibody
SKU/Catalog Number
PA1654-1
BA3695 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Amyloid-like protein 2 APLP2 Antibody catalog # PA1654-1. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Amyloid-like protein 2 APLP2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1654-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human APLP2, different from the related rat and mouse sequences by one amino acid.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1654-1 is reactive to APLP2 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
100-110 kDa
Calculated molecular weight
86956 MW
Background of APLP-2
APLP2 (Amyloid beta (A4) precursor-like protein 2), also known as CDEBP or SPERM MEMBRANE PROTEIN, is a protein that in humans is encoded by the APLP2 gene. The APLP2 gene is mapped on 11q24.3. The human amyloid precursor-like protein APLP2 is a highly conserved homolog of a sequence-specific DNA-binding mouse protein with an important function in the cell cycle. APLP2 along with APLP1 are important modulators of glucose and insulin homeostasis. APLP2 associates with antigen presentation molecules like MHC class I molecules and regulates their surface expression by enhancing endocytosis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1654-1 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Positive Control
WB: human SH-SY5Y whole cell, human HepG2 whole cell, human Hela whole cell, rat brain tissue, mouse brain tissue
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of APLP2 using anti-APLP2 antibody (PA1654-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human SH-SY5Y whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human Hela whole cell lysates,
Lane 4: rat brain tissue lysates,
Lane 5: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-APLP2 antigen affinity purified polyclonal antibody (Catalog # PA1654-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for APLP2 at approximately 100-110 kDa. The expected band size for APLP2 is at 87 kDa.
Protein Target Info & Infographic
Gene/Protein Information For APLP2 (Source: Uniprot.org, NCBI)
Gene Name
APLP2
Full Name
Amyloid-like protein 2
Weight
86956 MW
Superfamily
APP family
Alternative Names
Amyloid-like protein 2;APLP-2;APPH;Amyloid protein homolog;CDEI box-binding protein;CDEBP;APLP2;APPL2; APLP2 APLP-2, APPH, APPL2, CDEBP amyloid beta precursor like protein 2 amyloid-like protein 2|CDEI box-binding protein|amyloid beta (A4) precursor-like protein 2|amyloid precursor protein homolog HSD-2|testicular tissue protein Li 23
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on APLP2, check out the APLP2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for APLP2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Amyloid-like protein 2 APLP2 Antibody (PA1654-1)
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Customer Q&As
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7 Customer Q&As for Anti-Amyloid-like protein 2 APLP2 Antibody
Question
We are currently using anti-APLP2 antibody PA1654-1 for mouse tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2020-03-30
Answer
The anti-APLP2 antibody (PA1654-1) has not been tested for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-03-30
Question
Is a blocking peptide available for product anti-APLP2 antibody (PA1654-1)?
Verified Customer
Verified customer
Asked: 2019-10-23
Answer
We do provide the blocking peptide for product anti-APLP2 antibody (PA1654-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-10-23
Question
Does anti-APLP2 antibody PA1654-1 work for WB with ovary?
J. Krishna
Verified customer
Asked: 2019-07-03
Answer
According to the expression profile of ovary, APLP2 is highly expressed in ovary. So, it is likely that anti-APLP2 antibody PA1654-1 will work for WB with ovary.
Boster Scientific Support
Answered: 2019-07-03
Question
Here is the WB image, lot number and protocol we used for ovary using anti-APLP2 antibody PA1654-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-06-20
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-06-20
Question
Is there a BSA free version of anti-APLP2 antibody PA1654-1 available?
G. Roberts
Verified customer
Asked: 2019-03-25
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-APLP2 antibody PA1654-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-03-25
Question
I have a question about product PA1654-1, anti-APLP2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2017-06-01
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1654-1 anti-APLP2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-06-01
Question
I would like to test anti-APLP2 antibody PA1654-1 on rat ovary for research purposes, then I may be interested in using anti-APLP2 antibody PA1654-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
C. Banerjee
Verified customer
Asked: 2014-08-01
Answer
The products we sell, including anti-APLP2 antibody PA1654-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2014-08-01