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Product Info Summary
| SKU: | M01768 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Apg3 (Atg3) Rabbit Monoclonal Antibody
SKU/Catalog Number
M01768
BM5103 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Apg3 (Atg3) Rabbit Monoclonal Antibody catalog # M01768. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Apg3 (Atg3) Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01768)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
ABBC-1
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Apg3 (Atg3)
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01768 is reactive to ATG3 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
40 kDa
Calculated molecular weight
35864 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01768 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
Positive Control
WB: human Jurkat whole cell, human K562 whole cell, human Raji whole cell, monkey COS-7 whole cell, rat C6 whole cell, mouse kidney tissue, mouse NIH/3T3 whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Apg3 using anti-Apg3 antibody (M01768).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Jurkat whole cell lysates,
Lane 2: human K562 whole cell lysates,
Lane 3: human Raji whole cell lysates,
Lane 4: monkey COS-7 whole cell lysates,
Lane 5: rat C6 whole cell lysates,
Lane 6: mouse kidney tissue lysates,
Lane 7: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Apg3 antigen affinity purified monoclonal antibody (Catalog # M01768) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Apg3 at approximately 40 kDa. The expected band size for Apg3 is at 36 kDa.
Protein Target Info & Infographic
Gene/Protein Information For ATG3 (Source: Uniprot.org, NCBI)
Gene Name
ATG3
Full Name
Ubiquitin-like-conjugating enzyme ATG3
Weight
35864 MW
Superfamily
ATG3 family
Alternative Names
Ubiquitin-like-conjugating enzyme ATG3;6.3.2.-;Autophagy-related protein 3;APG3-like;hApg3;Protein PC3-96;ATG3;APG3, APG3L; ATG3 APG3, APG3-LIKE, APG3L, PC3-96 autophagy related 3 ubiquitin-like-conjugating enzyme ATG3|2610016C12Rik|APG3 autophagy 3-like|ATG3 autophagy related 3 homolog|autophagy-related protein 3|hApg3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ATG3, check out the ATG3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ATG3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Apg3 (Atg3) Rabbit Monoclonal Antibody (M01768)
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