Anti-Apelin/APLN Antibody Picoband®

Product Name

Anti-Apelin/APLN Antibody Picoband®

View all Apelin Antibodies

SKU/Catalog Number

PA1501

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Apelin/APLN Antibody catalog # PA1501. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Apelin/APLN Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1501)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human Apelin, identical to the related rat and mouse sequences.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1501 is reactive to APLN in Human, Mouse, Rat

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

78 kDa

Calculated molecular weight

8569 MW

Background of Apelin

Apelin is a neuropeptide expressed in the supraoptic and paraventricular nuclei that acts on specific receptors located on vasopressinergic neurons. The apelin gene to Xq25-q26.3 based on sequence similarity between the apelin sequence and a chromosome X PAC clone. This gene encodes a peptide that functions as an endogenous ligand for the G protein coupled receptor APJ. The encoded protein is synthesized as a prepropeptide that is processed into biologically active C-terminal fragments. The peptide fragments activate different tissue specific signaling pathways that regulate diverse biological functions including fluid homeostasis, cardiovascular function and insulin secretion. This protein also functions as a coreceptor for the human immunodeficiency virus 1.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

PA1501 is guaranteed for WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse

Positive Control

WB: U87 Cell, MCF-7 Cell, HELA Cell, MM453 Cell

Validation Images & Assay Conditions

Gene/Protein Information For APLN (Source: Uniprot.org, NCBI)

Alternative Names

Apelin;APJ endogenous ligand;Apelin-36;Apelin-31;Apelin-28;Apelin-13;APLN;APEL; APLN APEL, XNPEP2 apelin apelin|AGTRL1 ligand|APJ endogenous ligand

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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