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Product Info Summary
| SKU: | A10683-1 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-AP-B/RNPEP Antibody Picoband®
View all Aminopeptidase B/RNPEP Antibodies
SKU/Catalog Number
A10683-1
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-AP-B/RNPEP Antibody Picoband® catalog # A10683-1. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-AP-B/RNPEP Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10683-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human AP-B/RNPEP recombinant protein (Position: D66-K648).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A10683-1 is reactive to RNPEP in Human, Mouse
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
73 kDa
Calculated molecular weight
29370 MW
Background of Aminopeptidase B/RNPEP
Aminopeptidase B is an enzyme that in humans is encoded by the RNPEP gene. Predicted to enable metalloaminopeptidase activity. Predicted to be involved in proteolysis. Located in extracellular exosome.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A10683-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.25 μg/ml, Human, Mouse
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human Hela whole cell, human A549 whole cell, human HepG2 whole cell, human SiHa whole cell, mouse small intestine tissue, mouse stomach tissue
FCM: Hela cell, U251 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of AP-B/RNPEP using anti-AP-B/RNPEP antibody (A10683-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human A549 whole cell lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: human SiHa whole cell lysates,
Lane 5: mouse small intestine tissue lysates,
Lane 6: mouse stomach tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AP-B/RNPEP antigen affinity purified polyclonal antibody (Catalog # A10683-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AP-B/RNPEP at approximately 73 kDa. The expected band size for AP-B/RNPEP is at 73 kDa.
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Figure 2. Flow Cytometry analysis of Hela cells using anti-AP-B/RNPEP antibody (A10683-1).
Overlay histogram showing Hela cells stained with A10683-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-AP-B/RNPEP Antibody (A10683-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Figure 3. Flow Cytometry analysis of U251 cells using anti-AP-B/RNPEP antibody (A10683-1).
Overlay histogram showing U251 cells stained with A10683-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-AP-B/RNPEP Antibody (A10683-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For RNPEP (Source: Uniprot.org, NCBI)
Gene Name
RNPEP
Full Name
Aminopeptidase B
Weight
29370 MW
Superfamily
peptidase M1 family
Alternative Names
RNA-binding protein Nova-2; Astrocytic NOVA1-like RNA-binding protein; Neuro-oncological ventral antigen 2; NOVA2; ANOVA; NOVA3 RNPEP AP-B, APB arginyl aminopeptidase aminopeptidase B|arginine aminopeptidase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RNPEP, check out the RNPEP Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RNPEP: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-AP-B/RNPEP Antibody Picoband® (A10683-1)
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