Click image to see more details
Product Info Summary
| SKU: | A04671 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-ANKRD1 Antibody
SKU/Catalog Number
A04671
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-ANKRD1 Antibody (Catalog # A04671). Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ANKRD1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04671)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human ANKRD1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A04671 is reactive to ANKRD1 in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
36252 MW
Background of ANKRD1
May play an important role in endothelial cell activation. May act as a nuclear transcription factor that negatively regulates the expression of cardiac genes. Induction seems to be correlated with apoptotic cell death in hepatoma cells.
Chu W., J. Biol. Chem. 270:10236-10245(1995).
Park J.-H., Cancer Res. 65:2804-2814(2005).
Deloukas P., Nature 429:375-381(2004).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04671 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Validation Images & Assay Conditions
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Western blot analysis of extracts from COLO205 cells, using ANKRD1 antibody A04671.
Protein Target Info & Infographic
Gene/Protein Information For ANKRD1 (Source: Uniprot.org, NCBI)
Gene Name
ANKRD1
Full Name
Ankyrin repeat domain-containing protein 1
Weight
36252 MW
Alternative Names
Ankyrin repeat domain-containing protein 1;Cardiac ankyrin repeat protein;Cytokine-inducible gene C-193 protein;Cytokine-inducible nuclear protein;ANKRD1;C193, CARP, HA1A2; ANKRD1 ALRP, C-193, CARP, CVARP, MCARP, bA320F15.2 ankyrin repeat domain 1 ankyrin repeat domain-containing protein 1|ankyrin repeat domain 1 (cardiac muscle)|cardiac ankyrin repeat protein|cytokine-inducible gene C-193 protein|cytokine-inducible nuclear protein|epididymis secretory sperm binding protein|liver ankyrin repeat domain 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ANKRD1, check out the ANKRD1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ANKRD1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ANKRD1 Antibody (A04671)
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Customer Reviews
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Customer Q&As
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13 Customer Q&As for Anti-ANKRD1 Antibody
Question
We are currently using anti-ANKRD1 antibody A04671 for mouse tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2020-05-08
Answer
The anti-ANKRD1 antibody (A04671) has not been tested for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-05-08
Question
I would like to test anti-ANKRD1 antibody A04671 on human skin for research purposes, then I may be interested in using anti-ANKRD1 antibody A04671 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-12-27
Answer
The products we sell, including anti-ANKRD1 antibody A04671, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-12-27
Question
See below the WB image, lot number and protocol we used for skin using anti-ANKRD1 antibody A04671. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-07-05
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-07-05
Question
Can you help my question with product A04671, anti-ANKRD1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
J. Gonzalez
Verified customer
Asked: 2019-05-17
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A04671 anti-ANKRD1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-05-17
Question
Would anti-ANKRD1 antibody A04671 work on goat WB with skin?
Verified Customer
Verified customer
Asked: 2019-02-25
Answer
Our lab technicians have not tested anti-ANKRD1 antibody A04671 on goat. You can run a BLAST between goat and the immunogen sequence of anti-ANKRD1 antibody A04671 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat skin in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-02-25
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for skin using anti-ANKRD1 antibody A04671. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-02-04
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-02-04
Question
Is a blocking peptide available for product anti-ANKRD1 antibody (A04671)?
Verified Customer
Verified customer
Asked: 2019-01-10
Answer
We do provide the blocking peptide for product anti-ANKRD1 antibody (A04671). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-01-10
Question
I was wanting to use your anti-ANKRD1 antibody for WB for human skin on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human skin identification?
Verified Customer
Verified customer
Asked: 2018-10-30
Answer
It shows on the product datasheet, A04671 anti-ANKRD1 antibody has been validated for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human skin in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-10-30
Question
Is this A04671 anti-ANKRD1 antibody reactive to the isotypes of ANKRD1?
Verified Customer
Verified customer
Asked: 2018-10-25
Answer
The immunogen of A04671 anti-ANKRD1 antibody is Synthesized peptide derived from internal of human ANKRD1. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-10-25
Question
Will anti-ANKRD1 antibody A04671 work for WB with skin?
G. Li
Verified customer
Asked: 2018-02-28
Answer
According to the expression profile of skin, ANKRD1 is highly expressed in skin. So, it is likely that anti-ANKRD1 antibody A04671 will work for WB with skin.
Boster Scientific Support
Answered: 2018-02-28
Question
I see that the anti-ANKRD1 antibody A04671 works with WB, what is the protocol used to produce the result images on the product page?
J. Miller
Verified customer
Asked: 2017-09-01
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2017-09-01
Question
Will A04671 anti-ANKRD1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2017-08-10
Answer
You can see on the product datasheet, A04671 anti-ANKRD1 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-08-10
Question
Do you have a BSA free version of anti-ANKRD1 antibody A04671 available?
A. Patel
Verified customer
Asked: 2014-05-09
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-ANKRD1 antibody A04671 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2014-05-09
