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Product Info Summary
| SKU: | A09862-1 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-AMSH-LP/STAMBPL1 Antibody Picoband®
SKU/Catalog Number
A09862-1
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-AMSH-LP/STAMBPL1 Antibody Picoband® catalog # A09862-1. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-AMSH-LP/STAMBPL1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09862-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human AMSH-LP/STAMBPL1 recombinant protein (Position: Q187-R436).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A09862-1 is reactive to STAMBPL1 in Human, Mouse
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
50 kDa
Calculated molecular weight
49.783kDa
Background of AMSH-LP
STAMBPL1 (STAM-binding protein-like 1), also known as AMSH-FP, AMSH-LP (associated molecule with the SH3 domain of STAM like protein) or ALMa, is a 436 amino acid protein that belongs to the peptidase M67C family, contains one MPN domain and a JAMM motif that is essential for protease activity. Existing as two alternatively spliced isoforms, STAMBPL1 is a ubiquitously expressed protein that binds two zinc ions per subunit and acts as a zinc metalloprotease that specifically cleaves Lys-63-linked polyubiquitin chains. The gene that encodes STAMBPL1 maps to human chromosome 10q23.31. Chromosome 10 contains over 800 genes and 135 million nucleotides, making up nearly 4.5% of the human genome. PTEN is an important tumor suppressor gene located on chromosome 10 and, when defective, causes a genetic predisposition to cancer development known as Cowden syndrome. The chromosome 10 encoded gene ERCC6 is important for DNA repair and is linked to Cockayne syndrome which is characterized by extreme photosensitivity and premature aging. Tetrahydrobiopterin deficiency and a number of syndromes involving defective skull and facial bone fusion are also linked to chromosome 10.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A09862-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Mouse
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human MCF-7 whole cell, mouse NIH/3T3 whole cell
FCM: 293T cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of AMSH-LP/STAMBPL1 using anti-AMSH-LP/STAMBPL1 antibody (A09862-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human MCF-7 whole cell lysates,
Lane 2: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AMSH-LP/STAMBPL1 antigen affinity purified polyclonal antibody (Catalog # A09862-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AMSH-LP/STAMBPL1 at approximately 50 kDa. The expected band size for AMSH-LP/STAMBPL1 is at 50 kDa.
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Figure 2. Flow Cytometry analysis of 293T cells using anti-AMSH-LP/STAMBPL1 antibody (A09862-1).
Overlay histogram showing 293T cells stained with A09862-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-AMSH-LP/STAMBPL1 Antibody (A09862-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For STAMBPL1 (Source: Uniprot.org, NCBI)
Gene Name
STAMBPL1
Full Name
AMSH-like protease
Weight
49.783kDa
Superfamily
peptidase M67C family
Alternative Names
Interleukin-36 alpha; FIL1 epsilon; Interleukin-1 epsilon; IL-1 epsilon; Interleukin-1 family member 6; IL-1F6; IL36A; FIL1E; IL1E; IL1F6 STAMBPL1 ALMalpha, AMSH-FP, AMSH-LP, bA399O19.2 STAM binding protein like 1 AMSH-like protease|associated molecule with the SH3 domain of STAM (AMSH) - Family Protein|associated molecule with the SH3 domain of STAM (AMSH) like protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on STAMBPL1, check out the STAMBPL1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for STAMBPL1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-AMSH-LP/STAMBPL1 Antibody Picoband® (A09862-1)
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