Product Info Summary
SKU: | PB9983 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Rat |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-AMACR Antibody Picoband®
View all alpha-Methylacyl-CoA Racemase/AMACR Antibodies
SKU/Catalog Number
PB9983
PB1036 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-AMACR Antibody Picoband® catalog # PB9983. Tested in WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-AMACR Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9983)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human AMACR, different from the related mouse and rat sequences by four amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
PB9983 is reactive to AMACR in Human, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
42 kDa
Calculated molecular weight
42387 MW
Background of alpha-Methylacyl-CoA Racemase/AMACR
Alpha-methylacyl-CoA racemase (AMACR) is a mitochondrial and peroxisomal enzyme. It encodes a racemase. The encoded enzyme interconverts pristanoyl-CoA and C27-bile acylCoAs between their (R)- and (S)-stereoisomers. The conversion to the (S)-stereoisomers is necessary for degradation of these substrates by peroxisomal beta-oxidation. Encoded proteins from this locus localize to both mitochondria and peroxisomes. Mutations in this gene may be associated with adult-onset sensorimotor neuropathy, pigmentary retinopathy, and adrenomyeloneuropathy due to defects in bile acid synthesis. Alternatively spliced transcript variants have been described. Read-through transcription also exists between this gene and the upstream neighboring C1QTNF3 (C1q and tumor necrosis factor related protein 3) gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9983 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Rat
Positive Control
WB: rat kidney tissuerat liver tissueHEPG2 whole cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of AMHR2 using anti-AMHR2 antibody (PB9983).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat kidney tissue lysates.
Lane 2: rat liver tissue lysates.
Lane 3: HEPG2 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AMHR2 antigen affinity purified polyclonal antibody (Catalog # PB9983) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AMHR2 at approximately 42 kDa. The expected band size for AMHR2 is at 42 kDa.
Protein Target Info & Infographic
Gene/Protein Information For AMACR (Source: Uniprot.org, NCBI)
Gene Name
AMACR
Full Name
Alpha-methylacyl-CoA racemase
Weight
42387 MW
Superfamily
CoA-transferase III family
Alternative Names
Alpha-methylacyl-CoA racemase;5.1.99.4;2-methylacyl-CoA racemase;AMACR; Amacr|Mac, Macr1|alpha-methylacyl-CoA racemase|alpha-methylacyl-CoA racemase|2-arylpropionyl-CoA epimerase|2-methylacyl-CoA racemase|alpha-methylacyl-Coenzyme A racemase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on AMACR, check out the AMACR Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for AMACR: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-AMACR Antibody Picoband® (PB9983)
Hello CJ!
PB9983 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
A metanephric adenoma of the kidney associated with polycythemia: A case report
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Customer Q&As
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5 Customer Q&As for Anti-AMACR Antibody Picoband®
Question
We are currently using anti-AMACR antibody PB9983 for rat tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, rat. Is it likely that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2020-04-21
Answer
The anti-AMACR antibody (PB9983) has not been tested for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-04-21
Question
See attached the WB image, lot number and protocol we used for kidney using anti-AMACR antibody PB9983. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-12-20
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-20
Question
I was wanting to use your anti-AMACR antibody for WB for human kidney on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human kidney identification?
Verified Customer
Verified customer
Asked: 2018-05-15
Answer
It shows on the product datasheet, PB9983 anti-AMACR antibody has been tested for WB on human, rat tissues. We have an innovator award program that if you test this antibody and show it works in human kidney in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-05-15
Question
I was wanting to use to test anti-AMACR antibody PB9983 on human kidney for research purposes, then I may be interested in using anti-AMACR antibody PB9983 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
A. Anderson
Verified customer
Asked: 2015-05-28
Answer
The products we sell, including anti-AMACR antibody PB9983, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2015-05-28
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for kidney using anti-AMACR antibody PB9983. Let me know if you need anything else.
D. Miller
Verified customer
Asked: 2013-01-08
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2013-01-08